Bioactive Properties of Nanofibres Based on Concentrated Collagen Hydrolysate Loaded with Thyme and Oregano Essential Oils
Mariana Daniela Berechet,
Carmen Gaidau,
Aleksandra Miletic,
Branka Pilic,
Maria Râpă,
Maria Stanca,
Lia-Mara Ditu,
Rodica Constantinescu,
Andrada Lazea-Stoyanova
Affiliations
Mariana Daniela Berechet
Division Leather and Footwear Research Institute, National Research and Development Institute for Textiles and Leather, 031215 Bucharest, Romania
Carmen Gaidau
Division Leather and Footwear Research Institute, National Research and Development Institute for Textiles and Leather, 031215 Bucharest, Romania
Aleksandra Miletic
Faculty of Technology, University of Novi Sad, 21102 Novi Sad, Serbia
Branka Pilic
Faculty of Technology, University of Novi Sad, 21102 Novi Sad, Serbia
Maria Râpă
Center for Research and Eco-Metallurgical Expertise (ECOMET UPB), Politehnica University of Bucharest, 313 Spl. Independentei, 060042 Bucharest, Romania
Maria Stanca
Division Leather and Footwear Research Institute, National Research and Development Institute for Textiles and Leather, 031215 Bucharest, Romania
Lia-Mara Ditu
Faculty of Biology, University of Bucharest, 60101 Bucharest, Romania
Rodica Constantinescu
Division Leather and Footwear Research Institute, National Research and Development Institute for Textiles and Leather, 031215 Bucharest, Romania
Andrada Lazea-Stoyanova
National Institute for Lasers, Plasma and Radiation Physics, 077125 Magurele, Romania
This research aimed to obtain biocompatible and antimicrobial nanofibres based on concentrated collagen hydrolysate loaded with thyme or oregano essential oils as a natural alternative to synthesis products. The essential oils were successfully incorporated using electrospinning process into collagen resulting nanofibres with diameter from 471 nm to 580 nm and porous structure. The presence of essential oils in collagen nanofibre mats was confirmed by Attenuated Total Reflectance -Fourier Transform Infrared Spectroscopy (ATR-FTIR), Ultraviolet–visible spectroscopy (UV–VIS) and antimicrobial activity. Scanning Electron Microscopy with Energy Dispersive Spectroscopy analyses allowed evaluating the morphology and constituent elements of the nanofibre networks. Microbiological tests performed against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Candida albicans showed that the presence of essential oils supplemented the new collagen nanofibres with antimicrobial properties. The biocompatibility of collagen and collagen with essential oils was assessed by in vitro cultivation with NCTC clone 929 of fibroblastic cells and cell viability measurement. The results showed that the collagen and thyme or oregano oil composites have no cytotoxicity up to concentrations of 1000 μg·mL−1 and 500 μg mL−1, respectively. Optimization of electrospinning parameters has led to the obtaining of new collagen electrospun nanofibre mats loaded with essential oils with potential use for wound dressings, tissue engineering or protective clothing.
