Diagnostic study on Herpes simplex-1

Abstract

The study was planned to evaluate the prevalence of HSV-1 infection, and the use of rhabdomyosarcoma and L20B as cell lines for the primary propagation of human herpes simplex1, by using modern diagnostic techniques. The study was involved in a collection of 60 samples from dermal lesions, randomly selected from a population of ages ranges from 15 to 45 years. These samples were collected during a period extended from February to September 2013. Primarily, these samples were investigated by RT-PCR technique directed to certify human herpes simplex-1 infections. Bosphore® HSV-1&2 Genotyping Kit v1(Anatolia gene works, Turkey) was used for the detection protocol. From a total of 47 HSV-1 positive samples in PCR step, 20 samples were cultured using two cell lines (Rhabdomyosarcoma (RD) and L20B cell lines) in an attempt for virus isolation and evaluation capability of these cells for HSV-1 propagation. Results of applied PCR revealed that HSV-1 DNA was correlated with 47(78.3%) positive of the total cases investigated. In the attempt to HSV-1 isolation, both RD and L20B cell lines demonstrated a specific HSV-1 cytopathic effect. Herpes simplex virus type 1 had been propagated in 19 (95%) of the 20 PCR positive isolates by RD cell line, while 17:20 (85%) HSV-1 isolates were positive on L20B cells. In conclusions: The thermal protocol for Bosphore® HSV-1 Genotyping Kit v1allows very rapid detection of HSV-1 DNA in dermal lesions. It is finding to be laborsaving and shows sufficient sensitivity. The RD and L20B cell lines are efficient as cell lines for the propagation of HSV-1, for that It is well recommended to achieve future therapeutic studies on HSV-1.