Acta Biochimica Polonica (Jul 2024)

The modulation of the hexosamine biosynthetic pathway impacts the localization of CD36 in macrophages

  • Karen Julissa Loaeza-Reyes,
  • Karen Julissa Loaeza-Reyes,
  • Edgar Zenteno,
  • Eleazar Ramírez-Hernández,
  • Roberta Salinas-Marin,
  • Adriana Moreno-Rodríguez,
  • Rafael Torres-Rosas,
  • Liliana Argueta-Figueroa,
  • Liliana Argueta-Figueroa,
  • Berenice Fernández-Rojas,
  • Socorro Pina-Canseco,
  • Alfonso E. Acevedo-Mascarúa,
  • Alicia Hernández-Antonio,
  • Yobana Pérez-Cervera,
  • Yobana Pérez-Cervera

DOI
https://doi.org/10.3389/abp.2024.13004
Journal volume & issue
Vol. 71

Abstract

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CD36 is a type 2 cell surface scavenger receptor expressed in various tissues. In macrophages, CD36 recognizes oxidized low-density lipoprotein (ox-LDL), which promotes the formation of foam cells, the first step toward an atherosclerotic arterial lesion. CD36 possesses a variety of posttranslational modifications, among them N-glycosylation and O-GlcNAc modification. Some of the roles of these modifications on CD36 are known, such as N-linked glycosylation, which provides proper folding and trafficking to the plasma membrane in the human embryonic kidney. This study aimed to determine whether variations in the availability of UDP-GlcNAc could impact Rab-5-mediated endocytic trafficking and, therefore, the cellular localization of CD36. These preliminary results suggest that the availability of the substrate UDP-GlcNAc, modulated in response to treatment with Thiamet G (TMG), OSMI-1 (O-GlcNAcylation enzymes modulators) or Azaserine (HBP modulator), influences the localization of CD36 in J774 macrophages, and the endocytic trafficking as evidenced by the regulatory protein Rab-5, between the plasma membrane and the cytoplasm.

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