Supplementation of Salvia hispanica L. seed ameliorated liver function enzymes, hyperlipidemia, and oxidative stress in high fat diet fed Swiss albino mice

Abstract

Background: Salvia hispanica L. (Chia) seed improves liver marker enzymes, lipid profile and antioxidant enzyme activity. Purpose: The aim of the research work was to evaluate the hepatic and antioxidant enzymes activity of chia seed supplementation in high fat diet fed mice. Methods: Liver marker enzymes, lipid profile, oxidative stress, antioxidant enzyme activity, and histology were evaluated for chia seed supplementation in high fat diet fed mice. Here, ALT, AST, and ALP were done for liver marker enzymes test. Cholesterol, triglyceride, HDL, and LDL were measured for lipid profiles. MDA, NO, AOPP, and MPO were done for oxidative stress test. Catalase and SOD were performed for measuring antioxidant enzyme activity. Hematoxylin and eosin and Prussian blue staining were done for histology purposes. Results: In high fat diet fed mice liver marker enzymes were significantly reduced by supplementation of chia seed. Lipid profiles like cholesterol, triglyceride, LDL concentration were declined and HDL concentration was increased significantly by chia seed supplementation in high fat diet fed mice. High fat diet increased oxidative stress markers such as MDA, NO, AOPP, and MPO which was significantly decreased by the supplementation of chia seed. Antioxidant enzyme activity and histology were also improved by the supplementation of chia seed. Conclusion: According to this study, chia seed supplementation can reduce liver fibrosis, hyperlipidemia, oxidative stress, and improve antioxidant enzyme activity in mice fed a high-fat diet.

Keywords

• Salvia hispanica
• Fibrosis
• Dyslipidemia
• Oxidative stress