Scientific Reports (Nov 2024)

Effect of silver ion and silicate group on the antibacterial and antifungal properties of nanosized hydroxyapatite

  • Nataliia D. Pinchuk,
  • Agata Piecuch,
  • Natalia Charczuk,
  • Paulina Sobierajska,
  • Sara Targonska,
  • Oleksii Bezkrovnyi,
  • Rafał Ogórek,
  • Yadong Wang,
  • Rafal J. Wiglusz

DOI
https://doi.org/10.1038/s41598-024-80303-7
Journal volume & issue
Vol. 14, no. 1
pp. 1 – 14

Abstract

Read online

Abstract Hydroxyapatite is one of the most widely used materials in biomedical applications in reparative and regenerative medicine. Doping of nanosized hydroxyapatite improves its bioactive properties, and thus, the synthesis of different types of nanohydroxyapatite with antimicrobial activity is a perspective route of modern materials science. In this study, undoped hydroxyapatite (HAp), hydroxyapatite doped with silver (HAp with 0.1, 0.5 and 1 mol% Ag+ ions), and silicate-substituted hydroxyapatite doped with silver (Si-HAp with 0.1, 0.5 and 1 mol% Ag+ ions) nanoparticles (NPs) were synthesized by microwave-assisted hydrothermal technique and sintered at 450 °C. The structural properties and composition of obtained hydroxyapatite NPs were investigated using X-ray powder diffraction (XRPD), Fourier-transformed infrared spectroscopy (FT-IR), and Energy-dispersive X-ray spectroscopy (EDS). The morphology of synthesized nanosized powders was detected using the high-resolution transmission electron microscopy (HRTEM) technique. The results of XRPD for all synthesized nanosized powders confirmed the presence of hydroxyapatite crystal structure. The FT-IR spectra confirmed the presence of functional groups characteristic of the hydroxyapatite structure. The EDS analysis of obtained materials has shown the presence of Ca, P, O, Si, and Ag elements. Significant differences in size and morphology of the obtained particles were found using HRTEM. The particles have an elongated, rod-like shape with subtle differences. Moreover, HAp doped with 1 mol% Ag+ ions and Si-HAp doped with 1 mol% Ag+ ions nanosized powders showed antibacterial activity in comparison to pure hydroxyapatite both against gram-positive and gram-negative bacterial strains (Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus epidermidis, Staphylococcus aureus, Enterococcus faecalis). These two types of hydroxyapatite NPs also demonstrated antifungal activity against reference strains of Candida albicans, Candida kruzei, and Candida tropicalis, with stronger activity observed for Si-HAp doped with silver.

Keywords