Genome-Wide Identification and Analysis of the EPF Gene Family in <i>Sorghum bicolor</i> (L.) Moench
Zhiyin Jiao,
Jinping Wang,
Yannan Shi,
Zhifang Wang,
Jing Zhang,
Qi Du,
Bocheng Liu,
Xinyue Jia,
Jingtian Niu,
Chun Gu,
Peng Lv
Affiliations
Zhiyin Jiao
Institute of Millet Crops, Hebei Academy of Agriculture and Forestry Sciences/Hebei Branch of National Sorghum Improvement Center/Key Laboratory of Genetic Improvement and Utilization for Featured Coarse Cereals (Co-Construction by Ministry and Province), Ministry of Agriculture and Rural Affairs/Key Laboratory of Minor Cereal Crops of Hebei Province, Shijiazhuang 050035, China
Jinping Wang
Institute of Millet Crops, Hebei Academy of Agriculture and Forestry Sciences/Hebei Branch of National Sorghum Improvement Center/Key Laboratory of Genetic Improvement and Utilization for Featured Coarse Cereals (Co-Construction by Ministry and Province), Ministry of Agriculture and Rural Affairs/Key Laboratory of Minor Cereal Crops of Hebei Province, Shijiazhuang 050035, China
Yannan Shi
Institute of Millet Crops, Hebei Academy of Agriculture and Forestry Sciences/Hebei Branch of National Sorghum Improvement Center/Key Laboratory of Genetic Improvement and Utilization for Featured Coarse Cereals (Co-Construction by Ministry and Province), Ministry of Agriculture and Rural Affairs/Key Laboratory of Minor Cereal Crops of Hebei Province, Shijiazhuang 050035, China
Zhifang Wang
Institute of Millet Crops, Hebei Academy of Agriculture and Forestry Sciences/Hebei Branch of National Sorghum Improvement Center/Key Laboratory of Genetic Improvement and Utilization for Featured Coarse Cereals (Co-Construction by Ministry and Province), Ministry of Agriculture and Rural Affairs/Key Laboratory of Minor Cereal Crops of Hebei Province, Shijiazhuang 050035, China
Jing Zhang
Hebei Seed Management Station, Shijiazhuang 050031, China
Qi Du
Institute of Millet Crops, Hebei Academy of Agriculture and Forestry Sciences/Hebei Branch of National Sorghum Improvement Center/Key Laboratory of Genetic Improvement and Utilization for Featured Coarse Cereals (Co-Construction by Ministry and Province), Ministry of Agriculture and Rural Affairs/Key Laboratory of Minor Cereal Crops of Hebei Province, Shijiazhuang 050035, China
Bocheng Liu
Institute of Millet Crops, Hebei Academy of Agriculture and Forestry Sciences/Hebei Branch of National Sorghum Improvement Center/Key Laboratory of Genetic Improvement and Utilization for Featured Coarse Cereals (Co-Construction by Ministry and Province), Ministry of Agriculture and Rural Affairs/Key Laboratory of Minor Cereal Crops of Hebei Province, Shijiazhuang 050035, China
Xinyue Jia
Institute of Millet Crops, Hebei Academy of Agriculture and Forestry Sciences/Hebei Branch of National Sorghum Improvement Center/Key Laboratory of Genetic Improvement and Utilization for Featured Coarse Cereals (Co-Construction by Ministry and Province), Ministry of Agriculture and Rural Affairs/Key Laboratory of Minor Cereal Crops of Hebei Province, Shijiazhuang 050035, China
Jingtian Niu
Institute of Millet Crops, Hebei Academy of Agriculture and Forestry Sciences/Hebei Branch of National Sorghum Improvement Center/Key Laboratory of Genetic Improvement and Utilization for Featured Coarse Cereals (Co-Construction by Ministry and Province), Ministry of Agriculture and Rural Affairs/Key Laboratory of Minor Cereal Crops of Hebei Province, Shijiazhuang 050035, China
Chun Gu
Hebei Xingtang County Agro-Technology Extension Center, Shijiazhuang 050600, China
Peng Lv
Institute of Millet Crops, Hebei Academy of Agriculture and Forestry Sciences/Hebei Branch of National Sorghum Improvement Center/Key Laboratory of Genetic Improvement and Utilization for Featured Coarse Cereals (Co-Construction by Ministry and Province), Ministry of Agriculture and Rural Affairs/Key Laboratory of Minor Cereal Crops of Hebei Province, Shijiazhuang 050035, China
The EPIDERMAL PATTERNING FACTOR (EPF) plays a crucial role in plant response to abiotic stress. While the EPF has been extensively studied in model plants such as Arabidopsis thaliana, there is a lack of research on identifying EPF genes in the whole sorghum genome and its response to drought stress. In this study, we employed bioinformatics tools to identify 12 EPF members in sorghum. Phylogenetic tree analysis revealed that SbEPFs can be categorized into four branches. Further examination of the gene structure and protein conservation motifs of EPF family members demonstrated the high conservation of the SbEPF sequence. The promoter region of SbEPFs was found to encompass cis-elements responsive to stress and plant hormones. Moreover, real-time fluorescence quantitative results indicated that the SbEPFs have a tissue-specific expression. Under drought stress treatment, most SbEPF members were significantly up-regulated, indicating their potential role in drought response. Our research findings establish a foundation for investigating the function of SbEPFs and offer candidate genes for stress-resistant breeding and enhanced production in sorghum.