Co-Streptavidin Precipitation (Co-SP) is a method to pull down protein partners of a protein of interest tagged with the streptavidin binding protein domain, and using streptavidin columns that specifically bind to Streptavidin Binding Protein (SBP) in order to test the protein-protein interactions. Proteins of interest to be tested for their interaction are artificially co-expressed in “easy to transfect” cells. Pull down proteins can be analyzed by western blot for suspected protein partner.
