Induction of granule and Purkinje cells from primary cultured mouse cerebellar progenitors

Abstract

Summary: The architecturally stereotypical structure of cerebellum is ideal for investigating the generation of neuronal diversity, but in vitro models for assessing early cerebellar progenitor differentiation were lacking. Here, we report a detailed protocol for long-term in vitro generation of Pax6+ granule cells and Calbindin+ Purkinje cells from common Sox2+ embryonic cerebellar progenitors. We describe the procedure for dissecting mouse cerebellar anlage, cell seeding, and tamoxifen-induced labeling of progenitor cells, followed by time-lapse video recording of clonal expansion and neuronal differentiation.For complete details on the use and execution of this protocol, please refer to Zhang et al. (2021).

Keywords

• Cell Biology
• Cell culture
• Microscopy
• Neuroscience
• Stem Cells
• Cell Differentiation