Novel risk score model for non-proliferative diabetic retinopathy based on untargeted metabolomics of venous blood

Xinyu Wang; Xinyu Wang; Shu Yang; Shu Yang; Shu Yang; Guangyan Yang; Guangyan Yang; Guangyan Yang; Jialong Lin; Pengfei Zhao; Jingyun Ding; Hongyan Sun; Ting Meng; Ming Ming Yang; Ming Ming Yang; Ming Ming Yang; Lin Kang; Lin Kang; Lin Kang; Lin Kang; Zhen Liang; Zhen Liang; Zhen Liang

doi:10.3389/fendo.2023.1180415

Frontiers in Endocrinology (Aug 2023)

Novel risk score model for non-proliferative diabetic retinopathy based on untargeted metabolomics of venous blood

Xinyu Wang,
Xinyu Wang,
Shu Yang,
Shu Yang,
Shu Yang,
Guangyan Yang,
Guangyan Yang,
Guangyan Yang,
Jialong Lin,
Pengfei Zhao,
Jingyun Ding,
Hongyan Sun,
Ting Meng,
Ming Ming Yang,
Ming Ming Yang,
Ming Ming Yang,
Lin Kang,
Lin Kang,
Lin Kang,
Lin Kang,
Zhen Liang,
Zhen Liang,
Zhen Liang

Affiliations

Xinyu Wang: Department of Geriatrics, Shenzhen People’s Hospital (The Second Clinical Medical College, Jinan University, The First Affiliated Hospital, Southern University of Science and Technology, Shenzhen, China
Xinyu Wang: Department of Nephrology, The First People's Hospital of Yunnan Province, The Affiliated Hospital of Kunming University of Science and Technology, Kunming, China
Shu Yang: Department of Geriatrics, Shenzhen People’s Hospital (The Second Clinical Medical College, Jinan University, The First Affiliated Hospital, Southern University of Science and Technology, Shenzhen, China
Shu Yang: Guangdong Provincial Clinical Research Center for Geriatrics, Shenzhen Clinical Research Center for Geriatrics, Shenzhen People’s Hospital (The Second Clinical Medical College, Jinan University, Shenzhen, China
Shu Yang: The First Affiliated Hospital, Southern University of Science and Technology), Shenzhen, China
Guangyan Yang: Department of Geriatrics, Shenzhen People’s Hospital (The Second Clinical Medical College, Jinan University, The First Affiliated Hospital, Southern University of Science and Technology, Shenzhen, China
Guangyan Yang: Guangdong Provincial Clinical Research Center for Geriatrics, Shenzhen Clinical Research Center for Geriatrics, Shenzhen People’s Hospital (The Second Clinical Medical College, Jinan University, Shenzhen, China
Guangyan Yang: The First Affiliated Hospital, Southern University of Science and Technology), Shenzhen, China
Jialong Lin: Department of Cardiovascular Medicine, The Fourth Affiliated Hospital of Guangzhou Medical University, Zengcheng District People’s Hospital of Guangzhou, Guangzhou, China
Pengfei Zhao: Department of Geriatrics, Shenzhen People’s Hospital (The Second Clinical Medical College, Jinan University, The First Affiliated Hospital, Southern University of Science and Technology, Shenzhen, China
Jingyun Ding: Department of Geriatric, Shenzhen Second People’s Hospital, First Affiliated Hospital of Shenzhen University, Shenzhen, China
Hongyan Sun: Department of Ophthalmology, Shenzhen People’s Hospital, The Second Clinical Medical College, Jinan University, The First Affiliated Hospital, Southern University of Science and Technology, Shenzhen, China
Ting Meng: Department of Ophthalmology, Shenzhen People’s Hospital, The Second Clinical Medical College, Jinan University, The First Affiliated Hospital, Southern University of Science and Technology, Shenzhen, China
Ming Ming Yang: Guangdong Provincial Clinical Research Center for Geriatrics, Shenzhen Clinical Research Center for Geriatrics, Shenzhen People’s Hospital (The Second Clinical Medical College, Jinan University, Shenzhen, China
Ming Ming Yang: The First Affiliated Hospital, Southern University of Science and Technology), Shenzhen, China
Ming Ming Yang: Department of Ophthalmology, Shenzhen People’s Hospital, The Second Clinical Medical College, Jinan University, The First Affiliated Hospital, Southern University of Science and Technology, Shenzhen, China
Lin Kang: Department of Geriatrics, Shenzhen People’s Hospital (The Second Clinical Medical College, Jinan University, The First Affiliated Hospital, Southern University of Science and Technology, Shenzhen, China
Lin Kang: Guangdong Provincial Clinical Research Center for Geriatrics, Shenzhen Clinical Research Center for Geriatrics, Shenzhen People’s Hospital (The Second Clinical Medical College, Jinan University, Shenzhen, China
Lin Kang: The First Affiliated Hospital, Southern University of Science and Technology), Shenzhen, China
Lin Kang: The Biobank of National Innovation Center for Advanced Medical Devices, Shenzhen People’s Hospital, Shenzhen, China
Zhen Liang: Department of Geriatrics, Shenzhen People’s Hospital (The Second Clinical Medical College, Jinan University, The First Affiliated Hospital, Southern University of Science and Technology, Shenzhen, China
Zhen Liang: Guangdong Provincial Clinical Research Center for Geriatrics, Shenzhen Clinical Research Center for Geriatrics, Shenzhen People’s Hospital (The Second Clinical Medical College, Jinan University, Shenzhen, China
Zhen Liang: The First Affiliated Hospital, Southern University of Science and Technology), Shenzhen, China

DOI: https://doi.org/10.3389/fendo.2023.1180415
Journal volume & issue: Vol. 14

Abstract

Read online

Background and PurposeNonproliferative diabetic retinopathy (NPDR) occurs in the early stages of Diabetic retinopathy (DR), and the study of its metabolic markers will help to prevent DR. Hence, we aimed to establish a risk score based on multiple metabolites through untargeted metabolomic analysis of venous blood from NPDR patients and diabetic non-DR patients.Experimental ApproachUntargeted metabolomics of venous blood samples from patients with NPDR, diabetes melitus without DR were performed using high-performance liquid chromatography-mass spectrometry.ResultsDetailed metabolomic evaluation showed distinct clusters of metabolites in plasma samples from patients with NPDR and diabetic non-DR patients. NPDR patients had significantly higher levels of phenylacetylglycine, L-aspartic acid, tiglylglycine, and 3-sulfinato-L-alaninate, and lower level of indolelactic acid, threonic acid, L-arginine (Arg), and 4-dodecylbenzenesulfonic acid compared to control. The expression profiles of these eight NPDR risk-related characteristic metabolites were analyzed using Cox regression to establish a risk score model. Subsequently, univariate and multivariate Cox regression analyses were used to determine that this risk score model was a predictor of independent prognosis for NPDR.ConclusionsUntargeted metabolome analysis of blood metabolites revealed unreported metabolic alterations in NPDR patients compared with those in diabetic non-DR patients or MH. In the venous blood, we identified depleted metabolites thA and Arg, indicating that they might play a role in NPDR development.

Published in Frontiers in Endocrinology

ISSN: 1664-2392 (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Medicine: Internal medicine: Specialties of internal medicine: Diseases of the endocrine glands. Clinical endocrinology
Website: https://www.frontiersin.org/journals/endocrinology

About the journal

Abstract

Keywords