Quantifying telomeric lncRNAs using PNA-labelled RNA-Flow FISH (RNA-Flow)

Iria González-Vasconcellos; María A. Cobos-Fernández; Michael J. Atkinson; José Fernandez-Piqueras; Javier Santos

doi:10.1038/s42003-022-03452-3

Communications Biology (May 2022)

Quantifying telomeric lncRNAs using PNA-labelled RNA-Flow FISH (RNA-Flow)

Iria González-Vasconcellos,
María A. Cobos-Fernández,
Michael J. Atkinson,
José Fernandez-Piqueras,
Javier Santos

Affiliations

Iria González-Vasconcellos: Genome Dynamics and Function Programme, Genome Decoding Research Unit, Centro de Biología Molecular Severo Ochoa (CBMSO)
María A. Cobos-Fernández: Genome Dynamics and Function Programme, Genome Decoding Research Unit, Centro de Biología Molecular Severo Ochoa (CBMSO)
Michael J. Atkinson: Chair of Radiation Biology, Technical University of Munich
José Fernandez-Piqueras: Genome Dynamics and Function Programme, Genome Decoding Research Unit, Centro de Biología Molecular Severo Ochoa (CBMSO)
Javier Santos: Genome Dynamics and Function Programme, Genome Decoding Research Unit, Centro de Biología Molecular Severo Ochoa (CBMSO)

DOI: https://doi.org/10.1038/s42003-022-03452-3
Journal volume & issue: Vol. 5, no. 1
pp. 1 – 7

Abstract

Read online

Telomeric-associated long non-coding RNAs TERRA and TERC can be quantified in culture cell lines and human PBMCs using peptide nucleic acid probes with flow cytometry (RNA-Flow).

Published in Communications Biology

ISSN: 2399-3642 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science: Biology (General)
Website: https://www.nature.com/commsbio/

About the journal