Generation of human induced pluripotent stem cell line (MUi026-A) from a patient with autosomal dominant polycystic kidney disease carrying PKD1 point mutation

Warun Maneepitasut; Wasinee Wongkummool; Pirut Tong-ngam; Kornkanok Promthep; Alisa Tubsuwan; Aung Khine Linn; Bunyong Phakdeekitcharoen; Suparerk Borwornpinyo; Narisorn Kitiyanant; Phetcharat Phanthong; Suradej Hongeng

Stem Cell Research (May 2021)

Generation of human induced pluripotent stem cell line (MUi026-A) from a patient with autosomal dominant polycystic kidney disease carrying PKD1 point mutation

Warun Maneepitasut,
Wasinee Wongkummool,
Pirut Tong-ngam,
Kornkanok Promthep,
Alisa Tubsuwan,
Aung Khine Linn,
Bunyong Phakdeekitcharoen,
Suparerk Borwornpinyo,
Narisorn Kitiyanant,
Phetcharat Phanthong,
Suradej Hongeng

Affiliations

Warun Maneepitasut: Molecular Medicine Program, Multidisciplinary Unit, Faculty of Science, Mahidol University, Bangkok 10400, Thailand
Wasinee Wongkummool: Stem Cell Research Group, Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom 73170, Thailand
Pirut Tong-ngam: Stem Cell Research Group, Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom 73170, Thailand
Kornkanok Promthep: Research Center for Neuroscience, Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom 73170, Thailand
Alisa Tubsuwan: Stem Cell Research Group, Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom 73170, Thailand
Aung Khine Linn: Excellent Center for Drug Discovery, Faculty of Science, Mahidol University, Bangkok 10400 Thailand
Bunyong Phakdeekitcharoen: Division of Nephrology, Department of Medicine, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok 10400, Thailand
Suparerk Borwornpinyo: Excellent Center for Drug Discovery, Faculty of Science, Mahidol University, Bangkok 10400 Thailand; Department of Biotechnology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand
Narisorn Kitiyanant: Stem Cell Research Group, Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom 73170, Thailand; Corresponding authors.
Phetcharat Phanthong: Department of Anatomy, Faculty of Science, Mahidol University, Bangkok 10400, Thailand; Corresponding authors.
Suradej Hongeng: Department of Pediatrics, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok 10400, Thailand

Journal volume & issue: Vol. 53
p. 102306

Abstract

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Autosomal dominant polycystic kidney disease (ADPKD) is one of the common genetic kidney disorders that are caused by mutations in PKD1 or PKD2 gene. In this report, the MUi026-A human induced pluripotent stem cell (hiPSC) line was established from the skin fibroblasts of a female ADPKD patient who had the PKD1 mutation with c.5878C > T. The iPSC line retained normal karyotype. The cells displayed embryonic stem cell-like characteristics with pluripotency marker expression and were able to differentiate into three germ layers.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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