Generation of human induced pluripotent stem cell line encoding for a genetically encoded voltage indicator Arclight A242

Rajesh Vivekanandan; Monika Szepes; Maria Elena Ricci Signorini; Denys Kravchenko; Johanna Kiefer; Steffen Berger; Veronika Fricke; Gudrun Göhring; Ina Gruh

Stem Cell Research (Feb 2023)

Generation of human induced pluripotent stem cell line encoding for a genetically encoded voltage indicator Arclight A242

Rajesh Vivekanandan,
Monika Szepes,
Maria Elena Ricci Signorini,
Denys Kravchenko,
Johanna Kiefer,
Steffen Berger,
Veronika Fricke,
Gudrun Göhring,
Ina Gruh

Affiliations

Rajesh Vivekanandan: Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Department of Cardiac, Thoracic, Transplantation and Vascular Surgery (HTTG), Hannover Medical School, Germany
Monika Szepes: Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Department of Cardiac, Thoracic, Transplantation and Vascular Surgery (HTTG), Hannover Medical School, Germany
Maria Elena Ricci Signorini: Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Department of Cardiac, Thoracic, Transplantation and Vascular Surgery (HTTG), Hannover Medical School, Germany
Denys Kravchenko: Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Department of Cardiac, Thoracic, Transplantation and Vascular Surgery (HTTG), Hannover Medical School, Germany
Johanna Kiefer: Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Department of Cardiac, Thoracic, Transplantation and Vascular Surgery (HTTG), Hannover Medical School, Germany
Steffen Berger: Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Department of Cardiac, Thoracic, Transplantation and Vascular Surgery (HTTG), Hannover Medical School, Germany
Veronika Fricke: Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Department of Cardiac, Thoracic, Transplantation and Vascular Surgery (HTTG), Hannover Medical School, Germany
Gudrun Göhring: Department of Human Genetics, Hannover Medical School, Germany
Ina Gruh: Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Department of Cardiac, Thoracic, Transplantation and Vascular Surgery (HTTG), Hannover Medical School, Germany; Corresponding author.

Journal volume & issue: Vol. 66
p. 102981

Abstract

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Genetically encoded voltage indicators (GEVIs) allow for monitoring membrane potential changes in neurons and cardiomyocytes (CMs) as an alternative to patch-clamp techniques. GEVIs facilitate non-invasive, high throughput screening of electrophysiological properties of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs). A dual transgenic hiPSC line with Arclight A242 (GEVI) and an antibiotic resistance cardiac selection cassette was successfully generated from an earlier established hiPSC line MHHi001-A. After cardiac differentiation and selection, purified populations of CMs with constitutive GEVI expression can be utilized for studying cardiac development, disease modeling, and drug testing.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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