Universitas Scientiarum (Dec 2007)

EVALUACIÓN DE LA PRODUCTIVIDAD DE TRES MEDIOS DE CULTIVO PARA LA RECUPERACIÓN DE Helicobacter pylori

  • J. Navarro-Hernández,
  • A.P. Perea- Triana,
  • J.A. Pineda-Méndez,
  • O. Díez-Arbeláez,
  • M. Mercado-Reyes,
  • A.A. Trespalacios-Rangel

Journal volume & issue
Vol. 12, no. Edición Especial III
pp. 79 – 86

Abstract

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Infection of Helicobacter pylori since its discovery by Australian doctors Barry Marshall and RobinWarren in 1982 has been one of the scientific phenomena of greater importance in world-wide biomedicalliterature. Since then, numerous investigations have been developed to study this bacterium because itplays a significant role in etiology and pathogenesis of diverse gastrointestinal diseases like chronicgastritis and peptic ulcer, in addition to being a factor of important risk for development of gastric cancer.Cultivation is the most specific method to diagnose this microorganism. Despite its sensibility it variesnotably in relation to different variables: collection, transport and storage of samples; cultivation mediaand incubation conditions. This work evaluated the productivity of three culture media for H. pylorirecovery by ecometric method. All culture media showed low productivity. ICA values were lower than2,5 on all cultivation media. Culture media with ICA values of low and mid production were: Agar ATS:7,6% and 92,3%, Agar BHI: 0% and 100%, Agar Brucella: 6,6% and 93,3%.

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