Frontiers in Microbiology (May 2018)
Lactobacillus frumenti Facilitates Intestinal Epithelial Barrier Function Maintenance in Early-Weaned Piglets
- Jun Hu,
- Jun Hu,
- Jun Hu,
- Lingli Chen,
- Lingli Chen,
- Lingli Chen,
- Wenyong Zheng,
- Wenyong Zheng,
- Wenyong Zheng,
- Min Shi,
- Min Shi,
- Min Shi,
- Liu Liu,
- Liu Liu,
- Liu Liu,
- Chunlin Xie,
- Chunlin Xie,
- Chunlin Xie,
- Xinkai Wang,
- Xinkai Wang,
- Xinkai Wang,
- Yaorong Niu,
- Yaorong Niu,
- Yaorong Niu,
- Qiliang Hou,
- Qiliang Hou,
- Qiliang Hou,
- Xiaofan Xu,
- Xiaofan Xu,
- Xiaofan Xu,
- Baoyang Xu,
- Baoyang Xu,
- Baoyang Xu,
- Yimei Tang,
- Yimei Tang,
- Yimei Tang,
- Shuyi Zhou,
- Shuyi Zhou,
- Shuyi Zhou,
- Yiqin Yan,
- Yiqin Yan,
- Yiqin Yan,
- Tao Yang,
- Tao Yang,
- Tao Yang,
- Libao Ma,
- Libao Ma,
- Libao Ma,
- Xianghua Yan,
- Xianghua Yan,
- Xianghua Yan
Affiliations
- Jun Hu
- State Key Laboratory of Agricultural Microbiology, College of Animal Sciences and Technology, Huazhong Agricultural University, Wuhan, China
- Jun Hu
- The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, China
- Jun Hu
- Hubei Provincial Engineering Laboratory for Pig Precision Feeding and Feed Safety Technology, Wuhan, China
- Lingli Chen
- State Key Laboratory of Agricultural Microbiology, College of Animal Sciences and Technology, Huazhong Agricultural University, Wuhan, China
- Lingli Chen
- The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, China
- Lingli Chen
- Hubei Provincial Engineering Laboratory for Pig Precision Feeding and Feed Safety Technology, Wuhan, China
- Wenyong Zheng
- State Key Laboratory of Agricultural Microbiology, College of Animal Sciences and Technology, Huazhong Agricultural University, Wuhan, China
- Wenyong Zheng
- The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, China
- Wenyong Zheng
- Hubei Provincial Engineering Laboratory for Pig Precision Feeding and Feed Safety Technology, Wuhan, China
- Min Shi
- State Key Laboratory of Agricultural Microbiology, College of Animal Sciences and Technology, Huazhong Agricultural University, Wuhan, China
- Min Shi
- The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, China
- Min Shi
- Hubei Provincial Engineering Laboratory for Pig Precision Feeding and Feed Safety Technology, Wuhan, China
- Liu Liu
- State Key Laboratory of Agricultural Microbiology, College of Animal Sciences and Technology, Huazhong Agricultural University, Wuhan, China
- Liu Liu
- The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, China
- Liu Liu
- Hubei Provincial Engineering Laboratory for Pig Precision Feeding and Feed Safety Technology, Wuhan, China
- Chunlin Xie
- State Key Laboratory of Agricultural Microbiology, College of Animal Sciences and Technology, Huazhong Agricultural University, Wuhan, China
- Chunlin Xie
- The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, China
- Chunlin Xie
- Hubei Provincial Engineering Laboratory for Pig Precision Feeding and Feed Safety Technology, Wuhan, China
- Xinkai Wang
- State Key Laboratory of Agricultural Microbiology, College of Animal Sciences and Technology, Huazhong Agricultural University, Wuhan, China
- Xinkai Wang
- The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, China
- Xinkai Wang
- Hubei Provincial Engineering Laboratory for Pig Precision Feeding and Feed Safety Technology, Wuhan, China
- Yaorong Niu
- State Key Laboratory of Agricultural Microbiology, College of Animal Sciences and Technology, Huazhong Agricultural University, Wuhan, China
- Yaorong Niu
- The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, China
- Yaorong Niu
- Hubei Provincial Engineering Laboratory for Pig Precision Feeding and Feed Safety Technology, Wuhan, China
- Qiliang Hou
- State Key Laboratory of Agricultural Microbiology, College of Animal Sciences and Technology, Huazhong Agricultural University, Wuhan, China
- Qiliang Hou
- The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, China
- Qiliang Hou
- Hubei Provincial Engineering Laboratory for Pig Precision Feeding and Feed Safety Technology, Wuhan, China
- Xiaofan Xu
- State Key Laboratory of Agricultural Microbiology, College of Animal Sciences and Technology, Huazhong Agricultural University, Wuhan, China
- Xiaofan Xu
- The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, China
- Xiaofan Xu
- Hubei Provincial Engineering Laboratory for Pig Precision Feeding and Feed Safety Technology, Wuhan, China
- Baoyang Xu
- State Key Laboratory of Agricultural Microbiology, College of Animal Sciences and Technology, Huazhong Agricultural University, Wuhan, China
- Baoyang Xu
- The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, China
- Baoyang Xu
- Hubei Provincial Engineering Laboratory for Pig Precision Feeding and Feed Safety Technology, Wuhan, China
- Yimei Tang
- State Key Laboratory of Agricultural Microbiology, College of Animal Sciences and Technology, Huazhong Agricultural University, Wuhan, China
- Yimei Tang
- The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, China
- Yimei Tang
- Hubei Provincial Engineering Laboratory for Pig Precision Feeding and Feed Safety Technology, Wuhan, China
- Shuyi Zhou
- State Key Laboratory of Agricultural Microbiology, College of Animal Sciences and Technology, Huazhong Agricultural University, Wuhan, China
- Shuyi Zhou
- The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, China
- Shuyi Zhou
- Hubei Provincial Engineering Laboratory for Pig Precision Feeding and Feed Safety Technology, Wuhan, China
- Yiqin Yan
- State Key Laboratory of Agricultural Microbiology, College of Animal Sciences and Technology, Huazhong Agricultural University, Wuhan, China
- Yiqin Yan
- The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, China
- Yiqin Yan
- Hubei Provincial Engineering Laboratory for Pig Precision Feeding and Feed Safety Technology, Wuhan, China
- Tao Yang
- State Key Laboratory of Agricultural Microbiology, College of Animal Sciences and Technology, Huazhong Agricultural University, Wuhan, China
- Tao Yang
- The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, China
- Tao Yang
- Hubei Provincial Engineering Laboratory for Pig Precision Feeding and Feed Safety Technology, Wuhan, China
- Libao Ma
- State Key Laboratory of Agricultural Microbiology, College of Animal Sciences and Technology, Huazhong Agricultural University, Wuhan, China
- Libao Ma
- The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, China
- Libao Ma
- Hubei Provincial Engineering Laboratory for Pig Precision Feeding and Feed Safety Technology, Wuhan, China
- Xianghua Yan
- State Key Laboratory of Agricultural Microbiology, College of Animal Sciences and Technology, Huazhong Agricultural University, Wuhan, China
- Xianghua Yan
- The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, China
- Xianghua Yan
- Hubei Provincial Engineering Laboratory for Pig Precision Feeding and Feed Safety Technology, Wuhan, China
- DOI
- https://doi.org/10.3389/fmicb.2018.00897
- Journal volume & issue
-
Vol. 9
Abstract
Increased intestinal epithelial barrier function damages caused by early weaning stress have adverse effects on swine health and feed utilization efficiency. Probiotics have emerged as the promising antibiotic alternatives used for intestinal barrier function damage prevention. Our previous data showed that Lactobacillus frumenti was identified as a predominant Lactobacillus in the intestinal microbiota of weaned piglets. However, whether the intestinal epithelial barrier function in piglets was regulated by L. frumenti is still unclear. Here, piglets received a PBS vehicle or PBS suspension (2 ml, 108 CFU/ml) containing the L. frumenti by oral gavage once a day during the period of 6–20 days of age prior to early weaning. Our data demonstrated that oral administration of L. frumenti significantly improved the intestinal mucosal integrity and decreased the serum endotoxin and D-lactic acid levels in early-weaned piglets (26 days of age). The intestinal tight junction proteins (including ZO-1, Occludin, and Claudin-1) were significantly up-regulated by L. frumenti administration. The serum immunoglobulin G (IgG) levels, intestinal secretory immunoglobulin A (sIgA) levels, and interferon-γ (IFN-γ) levels were significantly increased by L. frumenti administration. Furthermore, our data revealed that oral administration of L. frumenti significantly increased the relative abundances of health-promoting microbes (including L. frumenti, Lactobacillus gasseri LA39, Parabacteroides distasonis, and Kazachstania telluris) and decreased the relative abundances of opportunistic pathogens (including Desulfovibrio desulfuricans and Candida humilis). Functional alteration of the intestinal bacterial community by L. frumenti administration was characterized by the significantly increased fatty acids and protein metabolism and decreased diseases-associated metabolic pathways. These findings suggest that L. frumenti facilitates intestinal epithelial barrier function maintenance in early-weaned piglets and may be a promising antibiotic alternative used for intestinal epithelial barrier function damage prevention in mammals.
Keywords
- Lactobacillus frumenti
- early-weaned piglets
- intestinal epithelial barrier function
- intestinal microbiota
- PICRUSt