Production of fatty acid-derived valuable chemicals in synthetic microbes

Frontiers in Bioengineering and Biotechnology. 2014;2 DOI 10.3389/fbioe.2014.00078

 

Journal Homepage

Journal Title: Frontiers in Bioengineering and Biotechnology

ISSN: 2296-4185 (Online)

Publisher: Frontiers Media S.A.

LCC Subject Category: Technology: Chemical technology: Biotechnology

Country of publisher: Switzerland

Language of fulltext: English

Full-text formats available: PDF, HTML, XML

 

AUTHORS

Ai-Qun eYu (National University of Singapore)
Ai-Qun eYu (National University of Singapore)
Nina Kurniasih Pratomo Juwono (National University of Singapore)
Nina Kurniasih Pratomo Juwono (National University of Singapore)
Susanna Su Jan Leong (National University of Singapore)
Susanna Su Jan Leong (National University of Singapore)
Susanna Su Jan Leong (Singapore Institute of Technology)
Matthew Wook Chang (National University of Singapore)
Matthew Wook Chang (National University of Singapore)

EDITORIAL INFORMATION

Blind peer review

Editorial Board

Instructions for authors

Time From Submission to Publication: 12 weeks

 

Abstract | Full Text

Fatty acid derivatives, such as hydroxy fatty acids, fatty alcohols, fatty acid methyl/ethyl esters and fatty alka(e)nes, have a wide range of industrial applications including plastics, lubricants and fuels. Currently, these chemicals are obtained mainly through chemical synthesis, which is complex and costly, and their availability from natural biological sources is extremely limited. Metabolic engineering of microorganisms has provided a platform for effective production of these valuable biochemicals. Notably, synthetic biology-based metabolic engineering strategies have been extensively applied to refactor microorganisms for improved biochemical production. Here, we reviewed (i) the current status of metabolic engineering of microbes that produce fatty acid-derived valuable chemicals, and (ii) the recent progress of synthetic biology approaches that assist metabolic engineering, such as mRNA secondary structure engineering, sensor-regulator system, regulatable expression system, ultrasensitive input/output control system, and computer science-based design of complex gene circuits. Furthermore, key challenges and strategies were discussed. Finally, we concluded that synthetic biology provides useful metabolic engineering strategies for economically viable production of fatty acid-derived valuable chemicals in engineered microbes.