Phenolic Compounds from An Algerian Endemic Species of <i>Hypochaeris laevigata</i> var. <i>hipponensis</i> and Investigation of Antioxidant Activities
Nabila Souilah,
Zain Ullah,
Hamdi Bendif,
Kamel Medjroubi,
Tahar Hazmoune,
Tarek Hamel,
Mehmet Öztürk,
Gema Nieto,
Salah Akkal
Affiliations
Nabila Souilah
Laboratory of Optimization of Agricultural Production in Sub-Humid Zones (LOPAZS), Faculty of Science, University of Skikda, Skikda 21000, Algeria
Zain Ullah
Department of Chemistry, Faculty of Sciences, University of Muğla Sitki Koçman, Muğla 48000, Turkey
Hamdi Bendif
Department of Natural Sciences and Life, Faculty of Science, University of M’sila, M’sila 28000, Algeria
Kamel Medjroubi
Laboratory of Valorization of Natural Resources, Bioactive Molecules and Physicochemical and Biological Analyzes Department of Chemistry, Faculty of Exact Sciences, University of Constantine 1, Constantine 25000, Algeria
Tahar Hazmoune
Laboratory of Optimization of Agricultural Production in Sub-Humid Zones (LOPAZS), Faculty of Science, University of Skikda, Skikda 21000, Algeria
Tarek Hamel
Department of Natural Sciences and Life, Faculty of Science, University of Badji Mokhtar, Annaba 23000, Algeria
Mehmet Öztürk
Department of Chemistry, Faculty of Sciences, University of Muğla Sitki Koçman, Muğla 48000, Turkey
Gema Nieto
Department of Food Technology, Food Science and Nutrition, Faculty of Veterinary Sciences, Regional Campus of International Excellence “Campus Mare Nostrum”, Espinardo, 30071 Murcia, Spain
Salah Akkal
Laboratory of Valorization of Natural Resources, Bioactive Molecules and Physicochemical and Biological Analyzes Department of Chemistry, Faculty of Exact Sciences, University of Constantine 1, Constantine 25000, Algeria
Hypochaeris laevigata var. hipponensis (Asteraceae) is an endemic plant from Algeria. In the current study, we analyzed for the first time its chemical composition, especially phenolic constituents of dichloromethane (DCM), ethyl acetate (EA), and n-butanol (BuOH) fractionsof the aerial parts of Hypochaeris laevigata var. hipponensis by liquid chromatography-mass spectrometry (LC-MS/MS). The number of phenolic compounds detected in DCM, EA, and BuOH fractions were found to be 9, 20, and 15, respectively. More specifically, 12 phenolic acids were detected. Among them, quinic acid, chlorogenic acid, and caffeic acid were the most abundant ones. Meanwhile, only seven flavonoids were detected. Among them, rutin, apigetrin, and isoquercitrin were the major ones. We also determined the total phenolic and flavonoid contents, and fraction EA showed the highest values, followed by BuOH, and DCM fractions. Furthermore, the antioxidant action was dictated by five methods and the tested plant fractions demonstrated a noteworthy antioxidant action.
