Determining the genotype and allele frequencies of DDX25 genes in different Iranian ethnic groups

Abstract

Abstract Background and Objectives: DDX25 Gene is located on chromosome 11q24 ,consists of 14 exons and effective for testicular function and spermatogenesis.DDX25 gene allele frequency in each population may be different.This difference may be due to environmental pressures and geographic areas are different.The impact of gene plays in the process of gametogenesis and Since Iran less attention to this issue and it has been scientific fields, this study was investigated the prevalence of genotype DDX25 in seven people, Persians, Turks, Baluchis, Gilaki, Arabs, Afghans. Methods: In this study, DNA samples was extracted from 180 menby Salting out.Then, using Ase-I restriction enzyme to RFLP and PCR product was cut and the resulting components have been studied using electrophoresis. Result: In calculating the frequency of genotypes, 22/77% of the population have the genotype GG, 11/6% of genotype TT and 66/16% of patients had genotype GT. G allele frequency of 0.855 and 0.145 for allele T were equal. Conclusion: According to the calculations performed by Chi-square test and Pvalue 0.48 In the study population, the ethnic Persians, Kurds, Turks, Baluchis, Gilaki, there was no significant difference between genotypes and blaring Arab population was not in Hardy-Weinberg equilibrium.

Keywords

• genotype
• allele frequencies
• ddx25 gene
• ethnic groups