Frontiers in Microbiology (May 2019)
CgGCS, Encoding a Glucosylceramide Synthase, Is Required for Growth, Conidiation and Pathogenicity in Colletotrichum gloeosporioides
- Yimei Huang,
- Yimei Huang,
- Yimei Huang,
- Yimei Huang,
- Bin Li,
- Bin Li,
- Bin Li,
- Bin Li,
- Jian Yin,
- Qiaosong Yang,
- Qiaosong Yang,
- Qiaosong Yang,
- Ou Sheng,
- Ou Sheng,
- Ou Sheng,
- Guiming Deng,
- Guiming Deng,
- Guiming Deng,
- Chunyu Li,
- Chunyu Li,
- Chunyu Li,
- Chunhua Hu,
- Chunhua Hu,
- Chunhua Hu,
- Tao Dong,
- Tao Dong,
- Tao Dong,
- Tongxin Dou,
- Tongxin Dou,
- Tongxin Dou,
- Huijun Gao,
- Huijun Gao,
- Huijun Gao,
- Fangcheng Bi,
- Fangcheng Bi,
- Fangcheng Bi,
- Ganjun Yi,
- Ganjun Yi,
- Ganjun Yi
Affiliations
- Yimei Huang
- Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Guangzhou, China
- Yimei Huang
- Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization, Ministry of Agriculture, Guangzhou, China
- Yimei Huang
- Key Laboratory of Tropical and Subtropical Fruit Tree Research, Guangdong Province, Guangzhou, China
- Yimei Huang
- College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, China
- Bin Li
- Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Guangzhou, China
- Bin Li
- Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization, Ministry of Agriculture, Guangzhou, China
- Bin Li
- Key Laboratory of Tropical and Subtropical Fruit Tree Research, Guangdong Province, Guangzhou, China
- Bin Li
- College of Life Sciences, South China Agricultural University, Guangzhou, China
- Jian Yin
- School of Life Sciences, Sun Yat-sen University, Guangzhou, China
- Qiaosong Yang
- Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Guangzhou, China
- Qiaosong Yang
- Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization, Ministry of Agriculture, Guangzhou, China
- Qiaosong Yang
- Key Laboratory of Tropical and Subtropical Fruit Tree Research, Guangdong Province, Guangzhou, China
- Ou Sheng
- Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Guangzhou, China
- Ou Sheng
- Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization, Ministry of Agriculture, Guangzhou, China
- Ou Sheng
- Key Laboratory of Tropical and Subtropical Fruit Tree Research, Guangdong Province, Guangzhou, China
- Guiming Deng
- Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Guangzhou, China
- Guiming Deng
- Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization, Ministry of Agriculture, Guangzhou, China
- Guiming Deng
- Key Laboratory of Tropical and Subtropical Fruit Tree Research, Guangdong Province, Guangzhou, China
- Chunyu Li
- Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Guangzhou, China
- Chunyu Li
- Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization, Ministry of Agriculture, Guangzhou, China
- Chunyu Li
- Key Laboratory of Tropical and Subtropical Fruit Tree Research, Guangdong Province, Guangzhou, China
- Chunhua Hu
- Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Guangzhou, China
- Chunhua Hu
- Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization, Ministry of Agriculture, Guangzhou, China
- Chunhua Hu
- Key Laboratory of Tropical and Subtropical Fruit Tree Research, Guangdong Province, Guangzhou, China
- Tao Dong
- Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Guangzhou, China
- Tao Dong
- Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization, Ministry of Agriculture, Guangzhou, China
- Tao Dong
- Key Laboratory of Tropical and Subtropical Fruit Tree Research, Guangdong Province, Guangzhou, China
- Tongxin Dou
- Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Guangzhou, China
- Tongxin Dou
- Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization, Ministry of Agriculture, Guangzhou, China
- Tongxin Dou
- Key Laboratory of Tropical and Subtropical Fruit Tree Research, Guangdong Province, Guangzhou, China
- Huijun Gao
- Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Guangzhou, China
- Huijun Gao
- Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization, Ministry of Agriculture, Guangzhou, China
- Huijun Gao
- Key Laboratory of Tropical and Subtropical Fruit Tree Research, Guangdong Province, Guangzhou, China
- Fangcheng Bi
- Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Guangzhou, China
- Fangcheng Bi
- Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization, Ministry of Agriculture, Guangzhou, China
- Fangcheng Bi
- Key Laboratory of Tropical and Subtropical Fruit Tree Research, Guangdong Province, Guangzhou, China
- Ganjun Yi
- Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Guangzhou, China
- Ganjun Yi
- Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization, Ministry of Agriculture, Guangzhou, China
- Ganjun Yi
- Key Laboratory of Tropical and Subtropical Fruit Tree Research, Guangdong Province, Guangzhou, China
- DOI
- https://doi.org/10.3389/fmicb.2019.01016
- Journal volume & issue
-
Vol. 10
Abstract
Fungal glucosylceramide plays important role in cell division, hyphal formation and growth, spore germination and the modulation of virulence and has recently been considered as target for small molecule inhibitors. In this study, we characterized CgGCS, a protein encoding a glucosylceramide synthase (GCS) in Colletotrichum gloeosporioides. Disruption of CgGCS resulted in a severe reduction of mycelial growth and defects in conidiogenesis. Sphingolipid profile analysis revealed large decreases in glucosylceramide production in the mutant strains. Pathogenicity assays indicated that the ability of the ΔCgGCS mutants to invade both tomato and mango hosts was almost lost. In addition, the expression levels of many genes, especially those related to metabolism, were shown to be affected by the mutation of CgGCS via transcriptome analysis. Overall, our results demonstrate that C. gloeosporioides glucosylceramide is an important regulatory factor in fungal growth, conidiation, and pathogenesis in hosts.
Keywords
