In Vitro Evaluation of Acellular Collagen Matrices Derived from Porcine Pericardium: Influence of the Sterilization Method on Its Biological Properties
Rone Aparecido De Grandis,
Larissa Natiele Miotto,
Luis Eduardo Genaro,
Larissa Migliatti Polli,
Ana Maria de Guzzi Plepis,
Fabiana Tessari Rodrigues,
Virginia da Conceição Amaro Martins,
Leonardo Pereira Franchi,
Raquel Mantuaneli Scarel-Caminaga,
Ticiana Sidorenko de Oliveira Capote
Affiliations
Rone Aparecido De Grandis
Faculty of Pharmaceutical Sciences-FCFar, Paulista State University, Araraquara 14801-903, SP, Brazil
Larissa Natiele Miotto
Department of Morphology, Genetics, Orthodontic and Pediatric Dentistry, Araraquara School of Dentistry, Paulista State University, Araraquara 14801-903, SP, Brazil
Luis Eduardo Genaro
Department of Morphology, Genetics, Orthodontic and Pediatric Dentistry, Araraquara School of Dentistry, Paulista State University, Araraquara 14801-903, SP, Brazil
Larissa Migliatti Polli
Faculty of Pharmaceutical Sciences-FCFar, Paulista State University, Araraquara 14801-903, SP, Brazil
Ana Maria de Guzzi Plepis
Department of Chemistry and Molecular Physics, Institute of Chemistry of São Carlos, University of São Paulo, Sao Carlos 13566-590, SP, Brazil
Fabiana Tessari Rodrigues
Department of Chemistry and Molecular Physics, Institute of Chemistry of São Carlos, University of São Paulo, Sao Carlos 13566-590, SP, Brazil
Virginia da Conceição Amaro Martins
Department of Chemistry and Molecular Physics, Institute of Chemistry of São Carlos, University of São Paulo, Sao Carlos 13566-590, SP, Brazil
Leonardo Pereira Franchi
Department of Genetics, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Sao Carlos 13566-590, SP, Brazil
Raquel Mantuaneli Scarel-Caminaga
Department of Morphology, Genetics, Orthodontic and Pediatric Dentistry, Araraquara School of Dentistry, Paulista State University, Araraquara 14801-903, SP, Brazil
Ticiana Sidorenko de Oliveira Capote
Department of Morphology, Genetics, Orthodontic and Pediatric Dentistry, Araraquara School of Dentistry, Paulista State University, Araraquara 14801-903, SP, Brazil
The aim of this study were characterize acellular collagen matrices derived from porcine pericardium (PP) and to evaluate their properties after sterilization by ethylene oxide and gamma ray. PP matrices were subjected to alkaline hydrolysis (AH), and samples were characterized for biological stability, membrane thickness measurements, differential scanning calorimetry (DSC) and scanning electron microscopy (SEM). Subsequently, the matrices were frozen, lyophilized and sterilized by ethylene oxide or gamma radiation. For in vitro assays, CHO-K1 cell culture was used and evaluated for cytotoxicity, clonogenic survival assay, genotoxicity and mutagenicity. Analysis of variance (ANOVA) was used, followed by Dunnett’s post-test, with a significance level of 5%. After AH, there was no significant change in matrix thickness. The relative biodegradability of the material after implantation was observed. Morphology and dimensions had small changes after AH. As for cell viability, none of the tested matrices showed a statistically significant difference (p > 0.05; Dunnett) regardless of the sterilization method. Furthermore, it was found that PP matrices did not interfere with the proliferation capacity of CHO-K1 cells (p > 0.05; Dunnett). As for genotoxicity, when sterilized with ethylene oxide (NP, P12 and P24), it showed genotoxic potential, but it was not genotoxic when sterilized by gamma radiation. No mutagenic effects were observed in either group. PP-derived collagen matrices hydrolyzed at different times were not cytotoxic. It is concluded that the best method of sterilization is through gamma radiation, since no significant changes were observed in the properties of the PP matrices.
