OncoTargets and Therapy (Mar 2020)

miR-145-5p Regulates the Proliferation, Migration and Invasion in Cervical Carcinoma by Targeting KLF5

  • Cao H,
  • Pan G,
  • Tang S,
  • Zhong N,
  • Liu H,
  • Zhou H,
  • Peng Q,
  • Zou Y

Journal volume & issue
Vol. Volume 13
pp. 2369 – 2376


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Hui Cao, Guihua Pan, Shiqiang Tang, Ni Zhong, Huake Liu, Haizhi Zhou, Qin Peng, Yongbin Zou Department of Oncology, Chenzhou First People’s Hospital, Chenzhou, People’s Republic of ChinaCorrespondence: Yongbin ZouDepartment of Oncology, Chenzhou First People’s Hospital, 102 Luojiajing Road, Chenzhou 423000, People’s Republic of ChinaTel +86-13507357355 Email [email protected]: Cervical carcinoma (CC) is a serious threat to women’s health and few effective therapeutic methods have been discovered. The purpose of this study is to explore the underlying mechanism of miR-145-5p in CC.Methods: Bioinformatics methods were employed to analyze the gene expression data of CC from TCGA database. qRT-PCR was used to detect the expression of miR-145-5p and KLF5 in CC cells, and Western blot was employed for the examination of KLF5 protein level. The targeted relationship between miR-145-5p and KLF5 was verified by a dual-luciferase reporter assay. Moreover, CCK-8, wound healing assay and transwell invasion assay were used to analyze the effects of miR-145-5p overexpression or KLF5 silencing on the proliferation, migration and invasion of CC cells.Results: miR-145-5p was shown to be down-regulated in CC tissues and cells, while KLF5 was up-regulated. miR-145-5p could bind to the complementary sequence within the wild type KLF5 3ʹUTR rather than the mutant one. In addition, miR-145-5p could effectively down-regulate KLF5, in turn inhibiting the proliferation, migration and invasion of CC cells.Conclusion: miR-145-5p regulates the proliferation, migration and invasion of CC cells by targeting KLF5.Keywords: cervical carcinoma, miR-145-5p, KLF5, proliferation, migration, invasion