Plant regeneration in Vernicia fordii from apical bud
Huijuan Zhang,
Shifei Qin,
Hui Cao,
Qingwei Zhang,
Keming Luo
Affiliations
Huijuan Zhang
Chongqing Key Laboratory of Plant Resource Conservation and Germplasm Innovation, School of Life Sciences, Southwest University, Chongqing, 400715, China; Key Laboratory of Eco-environments of Three Gorges Reservoir Region, Ministry of Education, Southwest University, Chongqing, 400715, China
Shifei Qin
Chongqing Key Laboratory of Plant Resource Conservation and Germplasm Innovation, School of Life Sciences, Southwest University, Chongqing, 400715, China; Key Laboratory of Eco-environments of Three Gorges Reservoir Region, Ministry of Education, Southwest University, Chongqing, 400715, China
Hui Cao
Chongqing Key Laboratory of Plant Resource Conservation and Germplasm Innovation, School of Life Sciences, Southwest University, Chongqing, 400715, China; Key Laboratory of Eco-environments of Three Gorges Reservoir Region, Ministry of Education, Southwest University, Chongqing, 400715, China
Qingwei Zhang
Chongqing Key Laboratory of Plant Resource Conservation and Germplasm Innovation, School of Life Sciences, Southwest University, Chongqing, 400715, China; Key Laboratory of Eco-environments of Three Gorges Reservoir Region, Ministry of Education, Southwest University, Chongqing, 400715, China; Corresponding author.
Keming Luo
Chongqing Key Laboratory of Plant Resource Conservation and Germplasm Innovation, School of Life Sciences, Southwest University, Chongqing, 400715, China; Key Laboratory of Eco-environments of Three Gorges Reservoir Region, Ministry of Education, Southwest University, Chongqing, 400715, China; Corresponding author.
Vernicia fordii is a woody plant species with important economic value, its seeds could be used to produce biodiesel and the oil extracted from them serve many kinds of industrial applications. In this study, we established a regeneration system of V. fordii for the first time using apical buds as explants on the Murashige and Skoog (MS) medium supplemented with the plant growth regulators, 6-benzylaminopurine (6-BA) and 3-indolebutyric acid (IBA). The optimal condition for adventitious bud induction was MS medium containing 1.5 mg/L 6-BA and 0.01 mg/L IBA, which produced a satisfactory induction efficiency. In subculture, the MS medium containing 1.0 mg/L 6-BA and 0.01 mg/L IBA was the optimum selection when considering both proliferation rate and tissue growth status. The adventitious shoots inoculated on 1/2 MS medium containing 0.5 mg/L IBA could produce strong roots and 96.4% of the regenerated plants survived after transplanting.
