CRISPitope: A generic platform to model target antigens for adoptive T cell transfer therapy in mouse tumor models

Abstract

Summary: This protocol details the procedure for CRISPR-assisted insertion of epitopes (CRISPitope), a flexible approach for generating tumor cells expressing model CD8+ T cell epitopes fused to endogenously encoded gene products of choice. CRISPitope-engineered tumor cells can be recognized by T cell receptor-transgenic (TCRtg) CD8+ T cells that are widely used in immunology research. Using mice inoculated with CRISPitope-engineered tumor cells, researchers can investigate how the choice of the target antigen for T cell immunotherapies influences treatment efficacy and resistance mechanisms.For complete details on the use and execution of this protocol, please refer to Effern et al. (2020).

Keywords

• Cell Biology
• Flow Cytometry/Mass Cytometry
• Cancer
• Immunology
• Model Organisms
• Molecular Biology