Graphene Oxide-Sensitized Surface Plasmon Resonance Biosensor of Porcine Reproductive and Respiratory Syndrome Virus
Xuemei Liu,
Chao Xu,
Chunyu Fu,
Dongfang Xia,
Fuchao Wang,
Hongzong Yin,
Jun Peng
Affiliations
Xuemei Liu
Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Veterinary Medicine, Shandong Agricultural University, Taian 271018, China
Chao Xu
College of Chemistry and Material Science, Shandong Agricultural University, Taian 271018, China
Chunyu Fu
Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Veterinary Medicine, Shandong Agricultural University, Taian 271018, China
Dongfang Xia
College of Chemistry and Material Science, Shandong Agricultural University, Taian 271018, China
Fuchao Wang
College of Chemistry and Material Science, Shandong Agricultural University, Taian 271018, China
Hongzong Yin
College of Chemistry and Material Science, Shandong Agricultural University, Taian 271018, China
Jun Peng
Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Veterinary Medicine, Shandong Agricultural University, Taian 271018, China
Biosensor analysis based on the surface plasmon resonance (SPR) phenomenon enables label-free, highly sensitive analyte detection without prior sample purification or processing. However, potential applications of SPR biosensors in virus detection in biological samples remain to be explored. Owing to its excellent biocompatibility and abundance of hydroxyl and carboxyl functional groups, graphene oxide (GO) has been widely used as a biosensor of proteins and metal ions in living cells. The present work explored the effect of GO modification on the sensitivity of an SPR biosensor and used a GO-modified sensor to detect porcine reproductive and respiratory syndrome virus in cell culture, as shown. The GO modification markedly enhanced the sensitivity of the Fourier transform SPR sensor and enabled linear detection of porcine reproductive and respiratory syndrome virus (PRRSV) with a multiplicity of infection in the range 0.2–1.7 (R2 = 0.998). Such a GO-modified sensor provides a promising alternative for virus detection.
