Docking-guided rational engineering of a macrolide glycosyltransferase glycodiversifies epothilone B

Abstract

Zhang et al. identify three hot regions of macrolide glycosyltransferase BsGT-1, that can play a role in epothilone B and sugar donor specificity. Further mutagenesis of BsGT-1 showed that specificity could be engineered and when the mutations were introduced into homologous GTs, promiscuity was improved, setting the stage for further rational glycodiversification of macrolides.