Molecular basis of regio- and stereo-specificity in biosynthesis of bacterial heterodimeric diketopiperazines

Chenghai Sun; Zhenyao Luo; Wenlu Zhang; Wenya Tian; Haidong Peng; Zhi Lin; Zixin Deng; Bostjan Kobe; Xinying Jia; Xudong Qu

doi:10.1038/s41467-020-20022-5

Nature Communications (Dec 2020)

Molecular basis of regio- and stereo-specificity in biosynthesis of bacterial heterodimeric diketopiperazines

Chenghai Sun,
Zhenyao Luo,
Wenlu Zhang,
Wenya Tian,
Haidong Peng,
Zhi Lin,
Zixin Deng,
Bostjan Kobe,
Xinying Jia,
Xudong Qu

Affiliations

Chenghai Sun: State Key Laboratory of Microbial Metabolism and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University
Zhenyao Luo: School of Chemistry and Molecular Biosciences, Institute for Molecular Bioscience and Australian Infectious Diseases Research Centre, The University of Queensland
Wenlu Zhang: Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, Wuhan University School of Pharmaceutical Sciences
Wenya Tian: State Key Laboratory of Microbial Metabolism and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University
Haidong Peng: Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, Wuhan University School of Pharmaceutical Sciences
Zhi Lin: State Key Laboratory of Microbial Metabolism and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University
Zixin Deng: State Key Laboratory of Microbial Metabolism and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University
Bostjan Kobe: School of Chemistry and Molecular Biosciences, Institute for Molecular Bioscience and Australian Infectious Diseases Research Centre, The University of Queensland
Xinying Jia: School of Chemistry and Molecular Biosciences, Institute for Molecular Bioscience and Australian Infectious Diseases Research Centre, The University of Queensland
Xudong Qu: State Key Laboratory of Microbial Metabolism and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University

DOI: https://doi.org/10.1038/s41467-020-20022-5
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 10

Abstract

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Bacterial heterodimeric tryptophan-containing diketopiperazines (HTDKPs) are bioactive natural products that are difficult to access chemically. Here, the authors identify a family of three related HTDKP-forming cytochrome P450s and engineer key amino acid residues to produce distinct diketopiperazines frameworks.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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