Наукові горизонти (Apr 2020)
LIPOPEROXIDATION AND ENZYMATIVE PROCESSES IN WINTER PEA PLANTS UNDER THE APPLICATION OF BIOLOGICALLY ACTIVE SUBSTANCES
Abstract
Herbicides are able to penetrate plants, where they undergo metabolic transformations (detoxification), as a result is changes in lipoperoxidation processes and enzymatic reactions. The article presents the results of the vegetative exeperiment to study on the influence of biologically active substances (herbicide, plant growth regulator and microbial preparation) on the way of reactions of lipid peroxidation and on the activity of glutathione-S-transferase enzyme in winter pea plants. The objects of the study were winter pea (Pisum sativum L.) of NS Moroz variety, MaxiMox herbicide (0,8; 0,9; 1,0 and 1,1 l/ha), applied separately and in mixtures with the Agriflex Amino plant growth regulator (1,0 kg/ha) with pre-sowing bacterization (and without pre-sowing bacterization ) of seeds by the microbical preparation Optimize Pulse (3,28 l/t). Analysis were performed on the third and tenth day after spraying the plants with preparations. The experiment was performed under strictly controlled conditions with the requirements of the vegetative method. During a research experiment, it was found that treatment of plants with the herbicide separately and in combination with the plant growth regulator and microbial preparation, the course of reactions of lipid peroxidation in winter pea plants changed significantly. In particular, the variants of complex used herbicide, plant growth regulator and microbial preparation showed the lowest level of lipid peroxidation (the content of malondialdehyde was lower by 14-27 % than in herbicide-based variants) with the highest activity of glutathione-S-transferase enzyme. This indicates a faster rate of neutralization of the toxicant, which generally contributed to the reduction level of lipid peroxidation on the background of the highest activity of glutathione-S-transferase enzyme.
Keywords
