<i>Lactiplantibacillus plantarum</i>-12 Alleviates Inflammation and Colon Cancer Symptoms in AOM/DSS-Treated Mice through Modulating the Intestinal Microbiome and Metabolome

Fenglian Ma; Mengying Sun; Yinglong Song; Arong Wang; Shujuan Jiang; Fang Qian; Guangqing Mu; Yanfeng Tuo

doi:10.3390/nu14091916

Nutrients (May 2022)

<i>Lactiplantibacillus plantarum</i>-12 Alleviates Inflammation and Colon Cancer Symptoms in AOM/DSS-Treated Mice through Modulating the Intestinal Microbiome and Metabolome

Fenglian Ma,
Mengying Sun,
Yinglong Song,
Arong Wang,
Shujuan Jiang,
Fang Qian,
Guangqing Mu,
Yanfeng Tuo

Affiliations

Fenglian Ma: School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China
Mengying Sun: School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China
Yinglong Song: School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China
Arong Wang: School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China
Shujuan Jiang: School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China
Fang Qian: School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China
Guangqing Mu: School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China
Yanfeng Tuo: School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China

DOI: https://doi.org/10.3390/nu14091916
Journal volume & issue: Vol. 14, no. 9
p. 1916

Abstract

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In our previous research, Lactiplantibacillus plantarum-12 alleviated inflammation in dextran sodium sulfate (DSS)-induced mice by regulating intestinal microbiota and preventing colon shortening (p L. plantarum-12 could ameliorate the colon cancer symptoms of azoxymethane (AOM)/DSS-treated C57BL/6 mice. The results showed that L. plantarum-12 alleviated colonic shortening (from 7.43 ± 0.15 to 8.23 ± 0.25) and weight loss (from 25.92 ± 0.21 to 27.75 ± 0.88) in AOM/DSS-treated mice. L. plantarum-12 oral administration down-regulated pro-inflammatory factors TNF-α (from 350.41 ± 15.80 to 247.72 ± 21.91), IL-8 (from 322.19 ± 11.83 to 226.08 ± 22.06), and IL-1β (111.43 ± 8.14 to 56.90 ± 2.70) levels and up-regulated anti-inflammatory factor IL-10 (from 126.08 ± 24.92 to 275.89 ± 21.87) level of AOM/DSS-treated mice. L. plantarum-12 oral administration restored the intestinal microbiota dysbiosis of the AOM/DSS treated mice by up-regulating beneficial Muribaculaceae, Lactobacillaceae, and Bifidobacteriaceae levels and down-regulating pathogenic Proteobacteria, Desulfovibrionaceae, and Erysipelotrichaceae levels. As a result, the fecal metabolites of the AOM/DSS-treated mice were altered, including xanthosine, uridine, 3,4-methylenesebacic acid, 3-hydroxytetradecanedioic acid, 4-hydroxyhexanoylglycine, beta-leucine, and glycitein, by L. plantarum-12 oral administration. Furthermore, L. plantarum-12 oral administration significantly ameliorated the colon injury of the AOM/DSS-treated mice by enhancing colonic tight junction protein level and promoting tumor cells death via down-regulating PCNA (proliferating cell nuclear antigen) and up-regulating pro-apoptotic Bax. (p L. plantarum-12 oral administration could ameliorate the colon cancer burden and inflammation of AOM-DSS-treated C57BL/6 mice through regulating the intestinal microbiota, manipulating fecal metabolites, enhancing colon barrier function, and inhibiting NF-κB signaling. These results suggest that L. plantarum-12 might be an excellent probiotic candidate for the prevention of colon cancer.

Published in Nutrients

ISSN: 2072-6643 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Technology: Home economics: Nutrition. Foods and food supply
Website: https://www.mdpi.com/journal/nutrients

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