Flora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi (Sep 2003)
Cloning of HBsAg Gene of Hepatitis B Virus in to an Expression Vector
Abstract
In the present study, the surface antigen (HBsAg) of HBV was amplified by polymerase chain reaction (PCR) and cloned into an expression vector. For this purpose, HBV DNA was obtained from a patient with chronic HBV infection. Then, the PCR product of HBsAg gene of HBV was cloned into pcDNA3.1/V5 HisTOPO vector. The resulting recombinant plasmid, termed pFHBsAg, was transformed into jM109 competent bacterial cells. The presence of the HBsAg gene was confirmed by PCR screening assay. Moreover, enzyme digestion assay was also performed for the same purpose. Both assays indicated that HBsAg gene of HBV was placed into the expression plasmid.
