A universal method for generating knockout mice in multiple genetic backgrounds using zygote electroporation

• Tomohiro Tamari,
• Yoshihisa Ikeda,
• Kento Morimoto,
• Keiko Kobayashi,
• Saori Mizuno-Iijima,
• Shinya Ayabe,
• Akihiro Kuno,
• Seiya Mizuno,
• Atsushi Yoshiki

Affiliations

Tomohiro Tamari

Model Generation & Breeding Service, The Jackson Laboratory Japan, Inc., 955 Kamibayashi, Ishioka, Ibaraki 315-0138, Japan

Yoshihisa Ikeda

Model Generation & Breeding Service, The Jackson Laboratory Japan, Inc., 955 Kamibayashi, Ishioka, Ibaraki 315-0138, Japan

Kento Morimoto

Doctoral Program in Medical Sciences, Graduate School of Comprehensive Human Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8575, Japan

Keiko Kobayashi

Model Generation & Breeding Service, The Jackson Laboratory Japan, Inc., 955 Kamibayashi, Ishioka, Ibaraki 315-0138, Japan

Saori Mizuno-Iijima

Experimental Animal Division, RIKEN BioResource Research Center, 3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan

Shinya Ayabe

Experimental Animal Division, RIKEN BioResource Research Center, 3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan

Akihiro Kuno

Department of Anatomy and Embryology, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8575, Japan

Seiya Mizuno

Laboratory Animal Resource Center in Trans-Border Medical Research Center, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8575, Japan

Atsushi Yoshiki

Experimental Animal Division, RIKEN BioResource Research Center, 3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan