Изучение структуры микробного сообщества черноземных почв Молдовы с использованием высокопроизводительного секвенирования

Abstract

Based on the new generation sequencing method – pyrosequencing (Illumina), for the first time in Moldova, there were determined and described the metagenomic characteristics of the typical chernozem microbiome under different land use conditions. It was revealed that the prokaryotic community is represented by both domains, in particular, seventeen bacterial phyla (59.8%) and two phylum Archaea (6.2%), only nine phyla had an abundance above 1.0%: the archaeal phylum Thaumarchaeotaand the bacterial phyla Proteobacteria, Actinobacteriota, Bacteroidota, Acidobacteriota, Firmicutes, Verrumicrobiota, Planctomycetota, and Myxococcota. Phyla with a share of less than 1.0% preferred the conditions of experimental variants of crop rotations. A significant proportion were unclassified microorganisms, the abundance of which varied from 25.8 to 38.2% in spring and from 27.9 to 40.2% in summer. The conducted studies showed that the agrotechnical measures had a significant impact on the structure of the microbiome community of a typical chernozem.

Keywords

• typical chernozem
• microbiome
• soil dna
• sequencing
• prokaryotae