Intra- and Interspecies Differences of Two <i>Cecropia</i> Species from Tabasco, Mexico, Determined through the Metabolic Analysis and <sup>1</sup>H-NMR-Based Fingerprinting of Hydroalcoholic Extracts
Eric Jaziel Medrano-Sánchez,
Gloria Ivonne Hernández-Bolio,
Carlos Ernesto Lobato-García,
Manasés González-Cortazar,
Mayra Antunez-Mojica,
Ammy Joana Gallegos-García,
Cristian Octavio Barredo-Hernández,
Ricardo López-Rodríguez,
Nelly Cristina Aguilar-Sánchez,
Abraham Gómez-Rivera
Affiliations
Eric Jaziel Medrano-Sánchez
División Académica de Ciencias Básicas, Universidad Juárez Autónoma de Tabasco, Carretera Cunduacán-Jalpa Km. 0.5, Cunduacán 86690, Tabasco, Mexico
Gloria Ivonne Hernández-Bolio
Departamento de Física Aplicada, Centro de Investigación y Estudios Avanzados del Instituto Politécnico Nacional, Unidad Mérida, Antigua Carretera a Progreso Km. 6, Mérida 97310, Yucatán, Mexico
Carlos Ernesto Lobato-García
División Académica de Ciencias Básicas, Universidad Juárez Autónoma de Tabasco, Carretera Cunduacán-Jalpa Km. 0.5, Cunduacán 86690, Tabasco, Mexico
Manasés González-Cortazar
Centro de Investigación Biomédica del Sur, Instituto Mexicano del Seguro Social, Argentina No. 1, Col. Centro, Xochitepec 62790, Morelos, Mexico
Mayra Antunez-Mojica
CONACYT-Centro de Investigaciones Químicas-IICBA, Universidad Autónoma del Estado de Morelos, Avenida Universidad 1001, Chamilpa, Cuernavaca 62209, Morelos, Mexico
Ammy Joana Gallegos-García
División de Ciencias Básicas e Ingeniería, Universidad Popular de la Chontalpa-Carretera Cárdenas-Huimanguillo Km 2 S/N, Ranchería, Invitab Paso y Playa, Heroica, Cárdenas 86556, Tabasco, Mexico
Cristian Octavio Barredo-Hernández
División Académica de Ciencias Básicas, Universidad Juárez Autónoma de Tabasco, Carretera Cunduacán-Jalpa Km. 0.5, Cunduacán 86690, Tabasco, Mexico
Ricardo López-Rodríguez
División Académica de Ciencias Básicas, Universidad Juárez Autónoma de Tabasco, Carretera Cunduacán-Jalpa Km. 0.5, Cunduacán 86690, Tabasco, Mexico
Nelly Cristina Aguilar-Sánchez
División Académica Multidisciplinaria de Jalpa de Méndez, Universidad Juárez Autónoma de Tabasco, Carretera Estatal Libre Villahermosa Comalcalco Km 27 S/N, Ranchería Rivera Alta, Jalpa de Méndez 86205, Tabasco, Mexico
Abraham Gómez-Rivera
División Académica de Ciencias Básicas, Universidad Juárez Autónoma de Tabasco, Carretera Cunduacán-Jalpa Km. 0.5, Cunduacán 86690, Tabasco, Mexico
The genus Cecropia is used in the traditional medicine of Tabasco, Mexico, in diabetes and hypertension treatments, mainly without distinction of the species. This contribution aimed to carry out the metabolic analysis and Proton Nuclear Magnetic Resonance (1H-NMR) spectroscopy-based fingerprinting of the hydroalcoholic leaf extracts of Cecropia peltata (Cp) and Cecropia obtusifolia (Co) collected in five sub-regions of the State of Tabasco (Cp1, “Centro”; Cp2, “Chontalpa”; Cp3, “Pantanos”; Cp4, “Ríos” and Co5, “Sierra”). Firstly, the extracts were evaluated for their Total Phenol Content (TPC) and Total Flavonoid Content (TFC) by spectrophotometric methods. In addition, metabolic analysis was performed using High-Performance Liquid Chromatography with Diode-Array Detection HPLC–DAD, which allowed the quantification of the chemical markers: chlorogenic acid, isoorientin, and orientin, as well as a vitexin analog. Finally, metabolomic analysis was carried out based on the 1H-NMR spectra. The Cp4 extract (C. peltata from the “Ríos” sub-region) presented the highest values of TPC (155 ± 9.1 mg GAE/g E) and TFC (724 ± 22.2 mg RE/g E). The metabolic analysis was similar among the five samples; the highest concentrations of the four chemical markers were found in Cp3 (C. peltata from the “Pantanos” sub-region) for chlorogenic acid (39.8 ± 2.3 mg/g) and isoorientin (51.5 ± 2.9 mg/g), in Cp4 for orientin (49.9 ± 0.6 mg/g), and in Cp2 (C. peltata from the “Chontalpa” sub-region) for the vitexin analog (6.2 ± 0.2 mg/g). The metabolic analysis and the 1H-NMR fingerprint analysis showed intraspecies differences among the C. peltata samples and interspecies between C. peltata and C. obtusifolia, which were attributed to variations in the metabolite groups as well as in the proportion of sugars such as glucose and xylose.
