BMC Biology (Apr 2021)
The reference genome and transcriptome of the limestone langur, Trachypithecus leucocephalus, reveal expansion of genes related to alkali tolerance
- Tengcheng Que,
- Huifeng Wang,
- Weifei Yang,
- Jianbao Wu,
- Chenyang Hou,
- Surui Pei,
- Qunying Wu,
- Liu Ming Li,
- Shilu Wei,
- Xing Xie,
- Hongli Huang,
- Panyu Chen,
- Yiming Huang,
- Aiqiong Wu,
- Meihong He,
- Dengpan Nong,
- Xiao Wei,
- Junyi Wu,
- Ru Nong,
- Ning Huang,
- Qingniao Zhou,
- Yaowang Lin,
- Tingxi Lu,
- Yongjie Wei,
- Shousheng Li,
- Jianglong Yao,
- Yanli Zhong,
- Huayong Qin,
- Luohao Tan,
- Yingjiao Li,
- Weidong Li,
- Tao Liu,
- Sanyang Liu,
- Yongyi Yu,
- Hong Qiu,
- Yonghua Jiang,
- Youcheng Li,
- Zhijin Liu,
- Cheng Ming Huang,
- Yanling Hu
Affiliations
- Tengcheng Que
- Terrestrial Wildlife Rescue and Epidemic Diseases Surveillance Center of Guangxi
- Huifeng Wang
- Department of Biochemistry and Molecular Biology, School of Pre-Clinical Medicine, Guangxi Medical University
- Weifei Yang
- Annoroad Gene Technology
- Jianbao Wu
- Guangxi Chongzuo white headed langur national nature reserve
- Chenyang Hou
- School of Information and Management, Guangxi Medical University
- Surui Pei
- Annoroad Gene Technology
- Qunying Wu
- Department of Biochemistry and Molecular Biology, School of Pre-Clinical Medicine, Guangxi Medical University
- Liu Ming Li
- Guangxi Reproductive Medical Research Center, First Affiliated Hospital of Guangxi Medical University
- Shilu Wei
- Life Sciences Institute, Guangxi Medical University
- Xing Xie
- Life Sciences Institute, Guangxi Medical University
- Hongli Huang
- Life Sciences Institute, Guangxi Medical University
- Panyu Chen
- Terrestrial Wildlife Rescue and Epidemic Diseases Surveillance Center of Guangxi
- Yiming Huang
- Center for Genomic and Personalized Medicine, Guangxi Medical University
- Aiqiong Wu
- Terrestrial Wildlife Rescue and Epidemic Diseases Surveillance Center of Guangxi
- Meihong He
- Terrestrial Wildlife Rescue and Epidemic Diseases Surveillance Center of Guangxi
- Dengpan Nong
- Terrestrial Wildlife Rescue and Epidemic Diseases Surveillance Center of Guangxi
- Xiao Wei
- Guangxi Chongzuo white headed langur national nature reserve
- Junyi Wu
- Nanning Animal Zoo
- Ru Nong
- Nanning Animal Zoo
- Ning Huang
- Nanning Animal Zoo
- Qingniao Zhou
- Department of Biochemistry and Molecular Biology, School of Pre-Clinical Medicine, Guangxi Medical University
- Yaowang Lin
- Department of Biochemistry and Molecular Biology, School of Pre-Clinical Medicine, Guangxi Medical University
- Tingxi Lu
- School of Information and Management, Guangxi Medical University
- Yongjie Wei
- Terrestrial Wildlife Rescue and Epidemic Diseases Surveillance Center of Guangxi
- Shousheng Li
- Terrestrial Wildlife Rescue and Epidemic Diseases Surveillance Center of Guangxi
- Jianglong Yao
- Terrestrial Wildlife Rescue and Epidemic Diseases Surveillance Center of Guangxi
- Yanli Zhong
- Terrestrial Wildlife Rescue and Epidemic Diseases Surveillance Center of Guangxi
- Huayong Qin
- Terrestrial Wildlife Rescue and Epidemic Diseases Surveillance Center of Guangxi
- Luohao Tan
- Terrestrial Wildlife Rescue and Epidemic Diseases Surveillance Center of Guangxi
- Yingjiao Li
- Terrestrial Wildlife Rescue and Epidemic Diseases Surveillance Center of Guangxi
- Weidong Li
- Life Sciences Institute, Guangxi Medical University
- Tao Liu
- Annoroad Gene Technology
- Sanyang Liu
- Annoroad Gene Technology
- Yongyi Yu
- Annoroad Gene Technology
- Hong Qiu
- Annoroad Gene Technology
- Yonghua Jiang
- Center for Genomic and Personalized Medicine, Guangxi Medical University
- Youcheng Li
- Life Sciences Institute, Guangxi Medical University
- Zhijin Liu
- College of Life Sciences, Capital Normal University
- Cheng Ming Huang
- College of Life Sciences, Capital Normal University
- Yanling Hu
- Department of Biochemistry and Molecular Biology, School of Pre-Clinical Medicine, Guangxi Medical University
- DOI
- https://doi.org/10.1186/s12915-021-00998-2
- Journal volume & issue
-
Vol. 19,
no. 1
pp. 1 – 15
Abstract
Abstract Background Trachypithecus leucocephalus, the white-headed langur, is a critically endangered primate that is endemic to the karst mountains in the southern Guangxi province of China. Studying the genomic and transcriptomic mechanisms underlying its local adaptation could help explain its persistence within a highly specialized ecological niche. Results In this study, we used PacBio sequencing and optical assembly and Hi-C analysis to create a high-quality de novo assembly of the T. leucocephalus genome. Annotation and functional enrichment revealed many genes involved in metabolism, transport, and homeostasis, and almost all of the positively selected genes were related to mineral ion binding. The transcriptomes of 12 tissues from three T. leucocephalus individuals showed that the great majority of genes involved in mineral absorption and calcium signaling were expressed, and their gene families were significantly expanded. For example, FTH1 primarily functions in iron storage and had 20 expanded copies. Conclusions These results increase our understanding of the evolution of alkali tolerance and other traits necessary for the persistence of T. leucocephalus within an ecologically unique limestone karst environment.
Keywords
