Screening of Potential Angiotensin-Converting Enzyme-Inhibitory Peptides in Squid (<i>Todarodes pacificus</i>) Skin Hydrolysates: Preliminary Study of Its Mechanism of Inhibition

Abstract

Background: Hypertension has been identified as a significant risk factor for cardiovascular disease. Given the prevalence of the adverse effects of angiotensin-converting enzyme-inhibitory (ACEI) drugs, natural and effective alternatives to these medications need to be identified. Methods: An investigative study was conducted to assess the ACEI capacity and structural characteristics of enzymatic hydrolysates with varying molecular weights derived from squid skin. The amino acid sequences of the enzymatic digests were analyzed via Nano LC-MS/MS and screened for peptides with ACEI activity using an in silico analysis. Furthermore, molecular docking was employed to investigate the interaction between potential ACEI peptides and ACE. Results: TPSH-V (MW 2+. Conclusions: Squid skin constitutes a viable resource for the production of ACEI peptides.

Keywords

• squid skin
• ACEI peptide
• sequence identification
• molecular docking