New colorimetric method to determine catalase mimic activity

Abstract

A new colorimetric method was used to determine catalase mimic activities of manganese dioxide (MnO _2 ), iron oxide (Fe _2 O _3 ) nanoparticles were prepared by a hydrothermal method (autoclave), and its composite. The MnO _2 nanoparticles were annealed at different temperatures (250–700 °C), while MnO _2 : Fe _2 O _3 composite in the mole ratio of 3:1 annealed at 400 °C. The structures and surface morphology were characterized by FT-IR measurements, x-ray diffraction (XRD), Scanning Electron Microscopy (SEM), and Atomic Force Microscope (AFM). This new method succeeds to determine catalase mimic activity, and found the activity of the composite was lower than its activity of MnO _2 alone, in the same annealing temperature.

Keywords

• catalase mimics activity
• MnO2
• Fe2O3
• potassium permanganate