Data regarding transplant induced germinal center humoral autoimmunity
M. Saeed Qureshi,
Jawaher Alsughayyir,
Manu Chhabra,
Jason M. Ali,
Martin J. Goddard,
Chris Devine,
Thomas M. Conlon,
Michelle A. Linterman,
Reza Motallebzadeh,
Gavin J. Pettigrew
Affiliations
M. Saeed Qureshi
University of Cambridge, School of Clinical Medicine, Cambridge CB2 0QQ, UK
Jawaher Alsughayyir
University of Cambridge, School of Clinical Medicine, Cambridge CB2 0QQ, UK
Manu Chhabra
University of Cambridge, School of Clinical Medicine, Cambridge CB2 0QQ, UK
Jason M. Ali
University of Cambridge, School of Clinical Medicine, Cambridge CB2 0QQ, UK
Martin J. Goddard
Department of Pathology, Papworth Hospital, Papworth Everard, CB23 3RE, UK
Chris Devine
University of Cambridge, School of Clinical Medicine, Cambridge CB2 0QQ, UK
Thomas M. Conlon
University of Cambridge, School of Clinical Medicine, Cambridge CB2 0QQ, UK
Michelle A. Linterman
Laboratory of Lymphocyte Signalling and Development, Babraham Institute, Cambridge CB22 3AT, UK
Reza Motallebzadeh
Division of Surgery and Interventional Sciences, University College London, London NW3 2QG, UK; Centre for Transplantation, Department of Renal Medicine, University College London, London NW3 2QG, UK; Institute of Immunity and Transplantation, Faculty of Medical Sciences, University College London, London NW3 2QG, UK
Gavin J. Pettigrew
University of Cambridge, School of Clinical Medicine, Cambridge CB2 0QQ, UK; Corresponding author.
This data is related to the research article entitled “Germinal center humoral autoimmunity independently mediates progression of allograft vasculopathy” (Harper et al., 2016) [2]. The data presented here focuses on the humoral autoimmune response triggered by transferred allogeneic CD4 T cells and includes details on: (a) the recipient splenic germinal center (GC) response; (b) augmentation of humoral autoimmunity and accelerated heart allograft rejection following transplantation from donors primed against recipient; (c) flow cytometric analysis of donor and recipient CD4 T cells for signature markers of T follicular helper cell differentiation; (d) in vitro donor endothelial cell migration in response to column purified autoantibody from recipient sera; (e) analysis of development of humoral responses in recipients following adoptive transfer of donor CD4 T cells and; (f) the development of humoral autoimmunity in mixed haematopoietic chimeric mice.
