Test and Evaluation of the Made-in-China Quick TargSeq Fully Integrated Automated On-site Rapid DNA Detection Instrument
HAN Jun-ping,
WANG Ying-xi,
LIU Chao,
LIU Feng,
GUO Jin-rong,
ZHU Wei,
TANG Guang-feng,
LI Cai-xia,
WANG Xin,
JIAO Zhang-ping
Affiliations
HAN Jun-ping
Criminal Investigation Detachment, Chaoyang District Branch of Beijing Public Security Bureau, Beijing 100025, China
WANG Ying-xi
Forensic Science Service of Beijing Public Security Bureau, Beijing 100192, China
LIU Chao
Guangzhou Institute of Criminal Science and Technology, Guangzhou 510030, China
LIU Feng
Liaoning Institute of Criminal Science and Technology, Shenyang 110032, China
GUO Jin-rong
Science and Technology Division, Department of Public Security of Shanxi Province, Taiyuan 030001, China
ZHU Wei
Tianjin Institute of Criminal Science and Technology, Tianjin 300384, China
TANG Guang-feng
Institute of Criminal Science and Technology, Tai’an Public Security Bureau, Tai’an 271000, Shandong Province, China
LI Cai-xia
Key Laboratory of Forensic Genetics, Beijing Engineering Research Center of Crime Scene Evidence Examination, National Engineering Laboratory for Forensic Science, Institute of Forensic Science of China, Beijing 100038, China
WANG Xin
Criminal Investigation Detachment, Huaibei Public Security Bureau, Huaibei 235000, Anhui Province, China
JIAO Zhang-ping
Forensic Science Service of Beijing Public Security Bureau, Beijing 100192, China
ObjectiveTo verify the stability and accuracy of the made-in-China Quick TargSeq Fully Integrated Automated On-site Rapid DNA Detection Instrument （hereinafter referred to as Quick TargSeq DNA detection instrument） and evaluate its forensic application prospects.MethodsA total of 74 blind samples provided by the expert group of Criminal Investigation Bureau of the Ministry of Public Security （4 individuals, 20 samples） and five participating institutions （54 samples） were used to test on 5 Quick TargSeq DNA detection instruments and the accompanying microfluidic chip cartridges. The results were compared with the known genotypes to calculate the samples’ detection rate and genotyping accuracy.ResultsThe results of reproducibility obtained from 20 samples provided by the expert group on the 5 Quick TargSeq DNA detection instruments were all consistent with the known genotypes. The detection rate of the 74 samples was 100%, and the genotyping accuracy was 97.33%.ConclusionThe Quick TargSeq DNA detection instrument has good stability and high genotyping accuracy.
