Crop Breeding and Applied Biotechnology (Mar 2016)

Construction and characterization of a bacterial artificial chromosome library of the maize inbred line Qi319

  • Chun Hua Mu,
  • Yu Yang,
  • Fa Jun Zhang,
  • Wen Cai Li,
  • Shou Ping Lu,
  • Zhao Dong Meng,
  • Xia Liu,
  • Guang Cun Li

DOI
https://doi.org/10.1590/1984-70332016v16n1a4
Journal volume & issue
Vol. 16, no. 1
pp. 22 – 27

Abstract

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Zea mays L. has been the most cultivated crop and the crop with the largest yield in China since 2012. We constructed a bacterial artificial chromosome (BAC) library for the maize inbred line Qi319, which may be used as a key source for disease-resistant maize breeding in China. The BAC contains 270,720 clones, with an average insert size of 90 kb. The coverage of the library is about 10.43 genome equivalents when considering a haploid genome size of 2300 Mb, providing a 99.99% likelihood of isolating any maize gene or sequence in the library. An average of 12 clones were obtained by polymerase chain reaction screening by using primer pairs linked to the genes for resistance to maize southern rust and rough dwarf. The results indicate that the library can satisfy the requirements for recovering specific sequences. The library is available to researchers to whom it may be of interest.

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