Toxins (Feb 2013)

ESI-MS/MS Identification of a Bradykinin-Potentiating Peptide from Amazon Bothrops atrox Snake Venom Using a Hybrid Qq-oaTOF Mass Spectrometer

  • Leonardo A. Calderon,
  • Rodrigo G. Stábeli,
  • Juliana P. Zuliani,
  • Andreimar M. Soares,
  • Anderson M. Kayano,
  • Rodrigo S. Silva,
  • Kayena D. Zaqueo,
  • Gustavo H. M. F. Souza,
  • Cleópatra A. S. Caldeira,
  • Antonio Coutinho-Neto

DOI
https://doi.org/10.3390/toxins5020327
Journal volume & issue
Vol. 5, no. 2
pp. 327 – 335

Abstract

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A bradykinin-potentiating peptide (BPP) from Amazon Bothrops atrox venom with m/z 1384.7386 was identified and characterized by collision induced dissociation (CID) using an ESI-MS/MS spectra obtained in positive ion mode on a hybrid Qq-oaTOF mass spectrometer, Xevo G2 QTof MS (Waters, Manchester, UK). De novo peptide sequence analysis of the CID fragmentation spectra showed the amino acid sequence ZKWPRPGPEIPP, with a pyroglutamic acid and theoretical monoisotopic m/z 1384.7378, which is similar to experimental data, showing a mass accuracy of 0.6 ppm. The peptide is homologous to other BPP from Bothrops moojeni and was named as BPP-BAX12.

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