Generation of a healthy heavy smoker patient-derived induced pluripotent stem cell line UHOMi007-A from peripheral blood mononuclear cells

Engi Ahmed; Mathieu Fieldès; Chloé Bourguignon; Joffrey Mianné; Aurélie Petit; Nasri Amel; Florent Foisset; Carine Bourdais; Isabelle Vachier; Said Assou; John De Vos; Arnaud Bourdin

Stem Cell Research (Jun 2024)

Generation of a healthy heavy smoker patient-derived induced pluripotent stem cell line UHOMi007-A from peripheral blood mononuclear cells

Engi Ahmed,
Mathieu Fieldès,
Chloé Bourguignon,
Joffrey Mianné,
Aurélie Petit,
Nasri Amel,
Florent Foisset,
Carine Bourdais,
Isabelle Vachier,
Said Assou,
John De Vos,
Arnaud Bourdin

Affiliations

Engi Ahmed: IRMB, Univ Montpellier, INSERM 1183, CHU Montpellier, Montpellier 34000, France; Department of Respiratory Diseases, CHU Montpellier, Arnaud de Villeneuve Hospital, INSERM, Montpellier 34000, France
Mathieu Fieldès: IRMB, Univ Montpellier, INSERM 1183, CHU Montpellier, Montpellier 34000, France
Chloé Bourguignon: IRMB, Univ Montpellier, INSERM 1183, CHU Montpellier, Montpellier 34000, France
Joffrey Mianné: IRMB, Univ Montpellier, INSERM 1183, CHU Montpellier, Montpellier 34000, France
Aurélie Petit: Department of Respiratory Diseases, CHU Montpellier, Arnaud de Villeneuve Hospital, INSERM, Montpellier 34000, France
Nasri Amel: IRMB, Univ Montpellier, INSERM 1183, CHU Montpellier, Montpellier 34000, France
Florent Foisset: IRMB, Univ Montpellier, INSERM 1183, CHU Montpellier, Montpellier 34000, France
Carine Bourdais: IRMB, Univ Montpellier, INSERM 1183, CHU Montpellier, Montpellier 34000, France
Isabelle Vachier: Department of Respiratory Diseases, CHU Montpellier, Arnaud de Villeneuve Hospital, INSERM, Montpellier 34000, France; MedBioMed, Montpellier, France
Said Assou: IRMB, Univ Montpellier, INSERM 1183, CHU Montpellier, Montpellier 34000, France
John De Vos: IRMB, Univ Montpellier, INSERM 1183, CHU Montpellier, Montpellier 34000, France; Department of Cell and Tissue Engineering, Saint-Eloi Hospital, Montpellier 34000, France; Corresponding author at: UTC, Saint-Eloi Hospital, 80 Avenue Augustin Fliche, 34000 Montpellier, France.
Arnaud Bourdin: Department of Respiratory Diseases, CHU Montpellier, Arnaud de Villeneuve Hospital, INSERM, Montpellier 34000, France; PhyMedExp INSERM 1046, University of Montpellier, Montpellier, France; Corresponding author at: Department of Respiratory Diseases, Montpellier University Hospitals, Arnaud de Villeneuve Hospital, 371 Av. du Doyen Gaston Giraud, 34090 Montpellier, France.

Journal volume & issue: Vol. 77
p. 103437

Abstract

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Human pluripotent stem cells (hiPSC) represent a unique opportunity to model lung development and chronic bronchial diseases. We generated a hiPSC line from a highly characterized healthy heavy smoker male donor free from emphysema or tobacco related disease. Peripheral blood mononuclear cells (PBMCs) were reprogrammed using integration-free Sendai virus. The cell line had normal karyotype, expressed pluripotency hallmarks, and differentiated into the three primary germ layers.The reported UHOMi007-A iPSC line may be used as a control to model lung development, study human chronic bronchial diseases and drug testing.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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