Attenuation of allergic airway inflammation and hyperresponsiveness in a murine model of asthma by silver nanoparticles

Abstract

Hee Sun Park1,*, Keun Hwa Kim1,*, Sunhyae Jang1, Ji Won Park1, Hye Rim Cha1, Jeong Eun Lee1, Ju Ock Kim1, Sun Young Kim1, Choong Sik Lee2, Joo Pyung Kim3, Sung Soo Jung11Division of Pulmonology, Allergy and Critical Care Medicine, Department of Internal Medicine, Chungnam National University Medical School, Daejeon, Korea; 2Department of Pathology, Chungnam National University Medical School, Daejeon, Korea; 3Nanochemical Incorporation, *Contributed equally to this workAbstract: The use of silver in the past demonstrated the certain antimicrobial activity, though this has been replaced by other treatments. However, nanotechnology has provided a way of ­producing pure silver nanoparticles, and it shows cytoprotective activities and possible ­pro-healing properties. But, the mechanism of silver nanoparticles remains unknown. This study was aimed to investigate the effects of silver nanoparticles on bronchial inflammation and hyperresponsiveness. We used ovalbumin (OVA)-inhaled female C57BL/6 mice to evaluate the roles of silver nanoparticles and the related molecular mechanisms in allergic airway disease. In this study with an OVA-induced murine model of allergic airway disease, we found that the increased inflammatory cells, airway hyperresponsiveness, increased levels of IL-4, IL-5, and IL-13, and the increased NF-κB levels in lungs after OVA inhalation were significantly reduced by the administration of silver nanoparticles. In addition, we have also found that the increased intracellular reactive oxygen species (ROS) levels in bronchoalveolar lavage fluid after OVA inhalation were decreased by the administration of silver nanoparticles. These results indicate that silver nanoparticles may attenuate antigen-induced airway inflammation and hyperresponsiveness. And antioxidant effect of silver nanoparticles could be one of the molecular bases in the murine model of asthma. These findings may provide a potential molecular mechanism of silver nanoparticles in preventing or treating asthma.Keywords: allergic airway disease, NF-κB, oxidative stress, silver nanoparticles