High-Resolution Magic-Angle-Spinning NMR in Revealing Hepatoblastoma Hallmarks
Ljubica Tasic,
Nataša Avramović,
Milka Jadranin,
Melissa Quintero,
Danijela Stanisic,
Lucas G. Martins,
Tássia Brena Barroso Carneiro Costa,
Estela Novak,
Vicente Odone,
Maria Rivas,
Talita Aguiar,
Dirce Maria Carraro,
Isabela Werneck da Cunha,
Cecilia Maria Lima da Costa,
Mariana Maschietto,
Ana Krepischi
Affiliations
Ljubica Tasic
Chemical Biology Laboratory, Department of Organic Chemistry, Institute of Chemistry, University of Campinas (UNICAMP), Campinas 13083-970, SP, Brazil
Nataša Avramović
Institute of Medical Chemistry, Faculty of Medicine, University of Belgrade, 11000 Belgrade, Serbia
Milka Jadranin
Institute of Chemistry, Technology, and Metallurgy, Department of Chemistry, University of Belgrade, 11000 Belgrade, Serbia
Melissa Quintero
Chemical Biology Laboratory, Department of Organic Chemistry, Institute of Chemistry, University of Campinas (UNICAMP), Campinas 13083-970, SP, Brazil
Danijela Stanisic
Chemical Biology Laboratory, Department of Organic Chemistry, Institute of Chemistry, University of Campinas (UNICAMP), Campinas 13083-970, SP, Brazil
Lucas G. Martins
Chemical Biology Laboratory, Department of Organic Chemistry, Institute of Chemistry, University of Campinas (UNICAMP), Campinas 13083-970, SP, Brazil
Tássia Brena Barroso Carneiro Costa
Chemical Biology Laboratory, Department of Organic Chemistry, Institute of Chemistry, University of Campinas (UNICAMP), Campinas 13083-970, SP, Brazil
Estela Novak
Pediatric Cancer Institute (ITACI), Pediatric Department, Sao Paulo University Medical School, Sao Paulo 05403-901, SP, Brazil
Vicente Odone
Pediatric Cancer Institute (ITACI), Pediatric Department, Sao Paulo University Medical School, Sao Paulo 05403-901, SP, Brazil
Maria Rivas
Human Genome and Stem Cell Research Center, Department of Genetics and Evolutionary Biology, Institute of Biosciences, University of Sao Paulo, Sao Paulo 05508-090, SP, Brazil
Talita Aguiar
Human Genome and Stem Cell Research Center, Department of Genetics and Evolutionary Biology, Institute of Biosciences, University of Sao Paulo, Sao Paulo 05508-090, SP, Brazil
Dirce Maria Carraro
International Center for Research, A. C. Camargo Cancer Center, Sao Paulo 01509-010, SP, Brazil
Isabela Werneck da Cunha
Pathology Department, Rede D’OR-São Luiz, Sao Paulo 04321-120, SP, Brazil
Cecilia Maria Lima da Costa
Department of Pediatric Oncology, A. C. Camargo Cancer Center, Sao Paulo 01509-010, SP, Brazil
Mariana Maschietto
National Laboratory of Biosciences (LNBio), National Center for Research in Energy and Materials (CNPEM), Campinas 13083-100, SP, Brazil
Ana Krepischi
Human Genome and Stem Cell Research Center, Department of Genetics and Evolutionary Biology, Institute of Biosciences, University of Sao Paulo, Sao Paulo 05508-090, SP, Brazil
Cancer is one of the leading causes of death in children and adolescents worldwide; among the types of liver cancer, hepatoblastoma (HBL) is the most common in childhood. Although it affects only two to three individuals in a million, it is mostly asymptomatic at diagnosis, so by the time it is detected it has already advanced. There are specific recommendations regarding HBL treatment, and ongoing studies to stratify the risks of HBL, understand the pathology, and predict prognostics and survival rates. Although magnetic resonance imaging spectroscopy is frequently used in diagnostics of HBL, high-resolution magic-angle-spinning (HR-MAS) NMR spectroscopy of HBL tissues is scarce. Using this technique, we studied the alterations among tissue metabolites of ex vivo samples from (a) HBL and non-cancer liver tissues (NCL), (b) HBL and adjacent non-tumor samples, and (c) two regions of the same HBL samples, one more centralized and the other at the edge of the tumor. It was possible to identify metabolites in HBL, then metabolites from the HBL center and the border samples, and link them to altered metabolisms in tumor tissues, highlighting their potential as biochemical markers. Metabolites closely related to liver metabolisms such as some phospholipids, triacylglycerides, fatty acids, glucose, and amino acids showed differences between the tissues.
