Effects of Trehalose Supplementation on Lipid Composition of Rooster Spermatozoa Membranes in a Freeze/Thaw Protocol
Olga I. Stanishevskaya,
Yulia Silyukova,
Elena Fedorova,
Nikolai Pleshanov,
Anton Kurochkin,
Vera M. Tereshina,
Elena Ianutsevich
Affiliations
Olga I. Stanishevskaya
Russian Research Institute of Farm Animal Genetics and Breeding—Branch of the LK Ernst Federal Research Center for Animal Husbandry, Moskovskoe Shosse, 55a, Pushkin, 196625 St. Petersburg, Russia
Yulia Silyukova
Russian Research Institute of Farm Animal Genetics and Breeding—Branch of the LK Ernst Federal Research Center for Animal Husbandry, Moskovskoe Shosse, 55a, Pushkin, 196625 St. Petersburg, Russia
Elena Fedorova
Russian Research Institute of Farm Animal Genetics and Breeding—Branch of the LK Ernst Federal Research Center for Animal Husbandry, Moskovskoe Shosse, 55a, Pushkin, 196625 St. Petersburg, Russia
Nikolai Pleshanov
Russian Research Institute of Farm Animal Genetics and Breeding—Branch of the LK Ernst Federal Research Center for Animal Husbandry, Moskovskoe Shosse, 55a, Pushkin, 196625 St. Petersburg, Russia
Anton Kurochkin
Russian Research Institute of Farm Animal Genetics and Breeding—Branch of the LK Ernst Federal Research Center for Animal Husbandry, Moskovskoe Shosse, 55a, Pushkin, 196625 St. Petersburg, Russia
Vera M. Tereshina
Winogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences, 119071 Moscow, Russia
Elena Ianutsevich
Winogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences, 119071 Moscow, Russia
The plasma membrane of spermatozoa plays an important role in the formation and maintenance of many functions of spermatozoa, including during cryopreservation. As a result of chromatographic analysis, the content of lipids and fatty acids in the membranes of spermatozoa of roosters of two breeds was determined under the influence of cryoprotective media containing trehalose LCM-control (0 mM), Treh20 (9.5 mM), and Treh30 (13.4 mM). The use of the cryoprotective diluent Treh20 made it possible to maintain a dynamic balance between the synthesis and degradation of phospholipids and sterols in the plasma membranes of frozen/thawed spermatozoa, close to that of native spermatozoa. This contributed to an increase in the preservation of frozen/thawed spermatozoa membranes from 48.3% to 52.2% in the egg breed and from 30.0% to 35.1% in the meat- and-egg breed. It was also noted that their kinetic apparatus (mobility indicators) remained at the level of 45.6% (egg breed) and 52.4% (meat-and-egg breed). An increase in the concentration of trehalose to 13.4 mM in a cryoprotective diluent for rooster sperm resulted in a decrease in the morphofunctional parameters of frozen/thawed spermatozoa.
