International Journal Bioautomation (Nov 2011)
Culture Media Optimization through Response Surface Methodology for in vitro Shoot Bud Development of Solanum melongena L. for Micropropagation
Abstract
Response surface methodology was used for the optimization of shoot bud response and shoot bud induction in leaf explants of Solanum melongena cultivar Arka Shirish. Three independent variables were evaluated for shoot bud response and shoot bud induction. The variables include the concentrations of nitrogen (N2), sucrose and growth regulator thidiazuron (TDZ). The shoot bud response for cultured explant was optimized at 4.34 g/l of total nitrogen, 2.65% of sucrose and 0.67 mg/l of TDZ with 95% response. The optimum medium conditions for shoot bud induction was found to be Murashige and Skoog (MS) basal medium supplemented with 4.02 g/l of total nitrogen, 2.36% of sucrose and 1.0 mg/l of TDZ with 10 number of bud per explant. The shoot buds so formed were elongated in 0.5 mg/l 2,3,5-Triiodobenzoic acid (TIBA) and 0.1 mg/l Gibberellic acid (GA3). The elongated shoots were rooted in MS with 1 mg/l Indole-3-butyric acid (IBA). The rooted plants were transferred to pots with farmyard manure upon hardening. This study has validation value for optimization of micropropagation protocol and is further useful in genetic transformation studies for Solanum melongena variety Arka Shirish to maximize regenerative response for automation.
