Artificial Cells, Nanomedicine, and Biotechnology (Dec 2024)

Haemostatic potency of sodium alginate/aloe vera/sericin composite scaffolds – preparation, characterisation, and evaluation

  • Jayavardhini Bhoopathy,
  • Weslen Vedakumari Sathyaraj,
  • Beryl Vedha Yesudhason,
  • Selvarajan Rajendran,
  • Sankari Dharmalingam,
  • Jayashri Seetharaman,
  • Ranjitha Muthu,
  • Ramachandran Murugesan,
  • Subramanian Raghunandhakumar,
  • Suresh Kumar Anandasadagopan

DOI
https://doi.org/10.1080/21691401.2023.2293784
Journal volume & issue
Vol. 52, no. 1
pp. 35 – 45

Abstract

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Fabrication of haemostatic materials with excellent antimicrobial, biocompatible and biodegradable properties remains as a major challenge in the field of medicine. Haemostatic agents play vital role in protecting patients and military individuals during emergency situations. Natural polymers serve as promising materials for fabricating haemostatic compounds due to their efficacy in promoting hemostasis and wound healing. In the present work, sodium alginate/aloe vera/sericin (SA/AV/S) scaffold has been fabricated using a simple cost-effective casting method. The prepared SA/AV/S scaffolds were characterised for their physicochemical properties such as scanning electron microscope, UV–visible spectroscopy and Fourier transform infra-red spectroscopy. SA/AV/S scaffold showed good mechanical strength, swelling behaviour and antibacterial activity. In vitro experiments using erythrocytes proved the hemocompatible and biocompatible features of SA/AV/S scaffold. In vitro blood clotting assay performed using human blood demonstrated the haemostatic and blood absorption properties of SA/AV/S scaffold. Scratch wound assay was performed to study the wound healing efficacy of prepared scaffolds. Chick embryo chorioallantoic membrane assay carried out using fertilised embryos proved the angiogenic property of SA/AV/S scaffold. Thus, SA/AV/S scaffold could serve as a potential haemostatic healthcare product due to its outstanding haemostatic, antimicrobial, hemocompatible, biocompatible and angiogenic properties.

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