PRMT1 promotes the proliferation and metastasis of gastric cancer cells by recruiting MLXIP for the transcriptional activation of the β-catenin pathway

Feng Wang; Shitong Chen; Shihan Peng; Xujun Zhou; Houyi Tang; Hanghua Liang; Xi Zhong; He Yang; Xiaoxue Ke; MuHan Lü; Hongjuan Cui

Genes and Diseases (Nov 2023)

PRMT1 promotes the proliferation and metastasis of gastric cancer cells by recruiting MLXIP for the transcriptional activation of the β-catenin pathway

Feng Wang,
Shitong Chen,
Shihan Peng,
Xujun Zhou,
Houyi Tang,
Hanghua Liang,
Xi Zhong,
He Yang,
Xiaoxue Ke,
MuHan Lü,
Hongjuan Cui

Affiliations

Feng Wang: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China; Cancer Center, Medical Research Institute, Southwest University, Chongqing 400716, China
Shitong Chen: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China; Cancer Center, Medical Research Institute, Southwest University, Chongqing 400716, China
Shihan Peng: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China; Cancer Center, Medical Research Institute, Southwest University, Chongqing 400716, China
Xujun Zhou: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China; Cancer Center, Medical Research Institute, Southwest University, Chongqing 400716, China
Houyi Tang: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China; Cancer Center, Medical Research Institute, Southwest University, Chongqing 400716, China
Hanghua Liang: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China; Cancer Center, Medical Research Institute, Southwest University, Chongqing 400716, China
Xi Zhong: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China; Cancer Center, Medical Research Institute, Southwest University, Chongqing 400716, China
He Yang: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China; Cancer Center, Medical Research Institute, Southwest University, Chongqing 400716, China
Xiaoxue Ke: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China; Cancer Center, Medical Research Institute, Southwest University, Chongqing 400716, China; Corresponding author. State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China.
MuHan Lü: Department of Gastroenterology, The Affiliated Hospital of Southwest Medical University, 25 Taiping Street, Jiangyang District, Luzhou, Sichuan 646000, China; Corresponding author.
Hongjuan Cui: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China; Cancer Center, Medical Research Institute, Southwest University, Chongqing 400716, China; Jinfeng Laboratory, Chongqing 401329, China; Corresponding author. State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China.

Journal volume & issue: Vol. 10, no. 6
pp. 2622 – 2638

Abstract

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Protein arginine methyltransferase 1 (PRMT1), a type I PRMT, is overexpressed in gastric cancer (GC) cells. To elucidate the function of PRMT1 in GC, PRMT1 expression in HGC-27 and MKN-45 cells was knocked down by short hairpin RNA (shRNA) or inhibited by PRMT1 inhibitors (AMI-1 or DCLX069), which resulted in inhibition of GC cell proliferation, migration, invasion, and tumorigenesis in vitro and in vivo. MLX-interacting protein (MLXIP) and Kinectin 1 (KTN1) were identified as PRMT1-binding proteins. PRMT1 recruited MLXIP to the promoter of β-catenin, which induced β-catenin transcription and activated the β-catenin signaling pathway, promoting GC cell migration and metastasis. Furthermore, KTN1 inhibited the K48-linked ubiquitination of PRMT1 by decreasing the interaction between TRIM48 and PRMT1. Collectively, our findings reveal a mechanism by which PRMT1 promotes cell proliferation and metastasis mediated by the β-catenin signaling pathway.

Published in Genes and Diseases

ISSN: 2352-3042 (Online)
Publisher: KeAi Communications Co., Ltd.
Country of publisher: China
LCC subjects: Medicine: Medicine (General); Science: Biology (General): Genetics
Website: https://www.keaipublishing.com/en/journals/genes-and-diseases/

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