Genome-Wide Investigation of N6-Methyladenosine Regulatory Genes and Their Roles in Tea (Camellia sinensis) Leaves During Withering Process

Chen Zhu; Chen Zhu; Chen Zhu; Chen Zhu; Shuting Zhang; Shuting Zhang; Chengzhe Zhou; Chengzhe Zhou; Chengzhe Zhou; Chengzhe Zhou; Siyi Xie; Siyi Xie; Siyi Xie; Guangwu Chen; Guangwu Chen; Guangwu Chen; Caiyun Tian; Caiyun Tian; Caiyun Tian; Kai Xu; Kai Xu; Kai Xu; Yuling Lin; Yuling Lin; Zhongxiong Lai; Zhongxiong Lai; Yuqiong Guo; Yuqiong Guo; Yuqiong Guo

doi:10.3389/fpls.2021.702303

Frontiers in Plant Science (Jun 2021)

Genome-Wide Investigation of N6-Methyladenosine Regulatory Genes and Their Roles in Tea (Camellia sinensis) Leaves During Withering Process

Chen Zhu,
Chen Zhu,
Chen Zhu,
Chen Zhu,
Shuting Zhang,
Shuting Zhang,
Chengzhe Zhou,
Chengzhe Zhou,
Chengzhe Zhou,
Chengzhe Zhou,
Siyi Xie,
Siyi Xie,
Siyi Xie,
Guangwu Chen,
Guangwu Chen,
Guangwu Chen,
Caiyun Tian,
Caiyun Tian,
Caiyun Tian,
Kai Xu,
Kai Xu,
Kai Xu,
Yuling Lin,
Yuling Lin,
Zhongxiong Lai,
Zhongxiong Lai,
Yuqiong Guo,
Yuqiong Guo,
Yuqiong Guo

Affiliations

Chen Zhu: College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, China
Chen Zhu: Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, Fuzhou, China
Chen Zhu: Key Laboratory of Tea Science in Universities of Fujian Province, Fujian Agriculture and Forestry University, Fuzhou, China
Chen Zhu: Tea Industry Research Institute, Fujian Agriculture and Forestry University, Fuzhou, China
Shuting Zhang: College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, China
Shuting Zhang: Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, Fuzhou, China
Chengzhe Zhou: College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, China
Chengzhe Zhou: Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, Fuzhou, China
Chengzhe Zhou: Key Laboratory of Tea Science in Universities of Fujian Province, Fujian Agriculture and Forestry University, Fuzhou, China
Chengzhe Zhou: Tea Industry Research Institute, Fujian Agriculture and Forestry University, Fuzhou, China
Siyi Xie: College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, China
Siyi Xie: Key Laboratory of Tea Science in Universities of Fujian Province, Fujian Agriculture and Forestry University, Fuzhou, China
Siyi Xie: Tea Industry Research Institute, Fujian Agriculture and Forestry University, Fuzhou, China
Guangwu Chen: College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, China
Guangwu Chen: Key Laboratory of Tea Science in Universities of Fujian Province, Fujian Agriculture and Forestry University, Fuzhou, China
Guangwu Chen: Tea Industry Research Institute, Fujian Agriculture and Forestry University, Fuzhou, China
Caiyun Tian: College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, China
Caiyun Tian: Key Laboratory of Tea Science in Universities of Fujian Province, Fujian Agriculture and Forestry University, Fuzhou, China
Caiyun Tian: Tea Industry Research Institute, Fujian Agriculture and Forestry University, Fuzhou, China
Kai Xu: College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, China
Kai Xu: Key Laboratory of Tea Science in Universities of Fujian Province, Fujian Agriculture and Forestry University, Fuzhou, China
Kai Xu: Tea Industry Research Institute, Fujian Agriculture and Forestry University, Fuzhou, China
Yuling Lin: College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, China
Yuling Lin: Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, Fuzhou, China
Zhongxiong Lai: College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, China
Zhongxiong Lai: Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, Fuzhou, China
Yuqiong Guo: College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, China
Yuqiong Guo: Key Laboratory of Tea Science in Universities of Fujian Province, Fujian Agriculture and Forestry University, Fuzhou, China
Yuqiong Guo: Tea Industry Research Institute, Fujian Agriculture and Forestry University, Fuzhou, China

DOI: https://doi.org/10.3389/fpls.2021.702303
Journal volume & issue: Vol. 12

Abstract

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N6-methyladenosine (m6A), one of the internal modifications of RNA molecules, can directly influence RNA abundance and function without altering the nucleotide sequence, and plays a pivotal role in response to diverse environmental stresses. The precise m6A regulatory mechanism comprises three types of components, namely, m6A writers, erasers, and readers. To date, the research focusing on m6A regulatory genes in plant kingdom is still in its infancy. Here, a total of 34 m6A regulatory genes were identified from the chromosome-scale genome of tea plants. The expansion of m6A regulatory genes was driven mainly by whole-genome duplication (WGD) and segmental duplication, and the duplicated gene pairs evolved through purifying selection. Gene structure analysis revealed that the sequence variation contributed to the functional diversification of m6A regulatory genes. Expression pattern analysis showed that most m6A regulatory genes were differentially expressed under environmental stresses and tea-withering stage. These observations indicated that m6A regulatory genes play essential roles in response to environmental stresses and tea-withering stage. We also found that RNA methylation and DNA methylation formed a negative feedback by interacting with each other’s methylation regulatory genes. This study provided a foundation for understanding the m6A-mediated regulatory mechanism in tea plants under environmental stresses and tea-withering stage.

Published in Frontiers in Plant Science

ISSN: 1664-462X (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Agriculture: Plant culture
Website: https://www.frontiersin.org/journals/plant-science

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