Comparative protective assessments of some antioxidants against cyclophosphamide-induced kidney toxicity in albino rats

Abstract

Introduction: Nephrotoxicity is one of the adverse effects of cyclophosphamide (CP). Objectives: The aim of this study is to comparatively investigate the protective effects of melatonin (MT), alpha-lipoic acid (ALA) and N-acetylcysteine (NAC) on CP-induced nephrotoxicity in albino rats. Materials and Methods: Sixty adult albino rats used for this study were divided into four groups (A-D). Rats in group A were treated with water intraperitoneally (ip) while rats in group B (B1-B4) were treated with NAC (10 mg/kg), ALA (10 mg/kg), MT (10 mg/kg) and MT+ALA ip respectively for 5 days. Rats in group C were treated with CP (150 mg/kg) ip on day 5. Rats in group D (D1- D4) were pretreated with NAC, MT, ALA and MT+ALA ip for 5 days before treatment with CP on day 5. Rats were sacrificed on the 6th day. Serum was extracted from blood and evaluated for renal function parameters. Kidneys were removed and used for light microscopic and biochemical studies. Results: CP-treated rats showed significant (P NAC>MT>ALA

Keywords

• Cyclophosphamide
• Kidney
• Toxicity
• Antioxidants