Ultrasound Assisted Extraction Approach to Test the Effect of Elastic Rubber Nettings on the N-Nitrosamines Content of Ham Meat Samples
Claudia Giménez-Campillo,
Marta Pastor-Belda,
Natalia Campillo,
Juan de Dios Hernández,
Isidro Guillén,
Pascuala Vizcaíno,
Ignacio López-García,
Manuel Hernández-Córdoba,
Natalia Arroyo-Manzanares,
Pilar Viñas
Affiliations
Claudia Giménez-Campillo
Department of Analytical Chemistry, Faculty of Chemistry, Regional Campus of International Excellence “Campus Mare Nostrum”, University of Murcia, E-30100 Murcia, Spain
Marta Pastor-Belda
Department of Analytical Chemistry, Faculty of Chemistry, Regional Campus of International Excellence “Campus Mare Nostrum”, University of Murcia, E-30100 Murcia, Spain
Natalia Campillo
Department of Analytical Chemistry, Faculty of Chemistry, Regional Campus of International Excellence “Campus Mare Nostrum”, University of Murcia, E-30100 Murcia, Spain
Juan de Dios Hernández
Productos del Sur S.A. (Prosur) Av. Francisco Salzillo, P/27-2, San Ginés, 30169 Murcia, Spain
Isidro Guillén
Productos del Sur S.A. (Prosur) Av. Francisco Salzillo, P/27-2, San Ginés, 30169 Murcia, Spain
Pascuala Vizcaíno
Productos del Sur S.A. (Prosur) Av. Francisco Salzillo, P/27-2, San Ginés, 30169 Murcia, Spain
Ignacio López-García
Department of Analytical Chemistry, Faculty of Chemistry, Regional Campus of International Excellence “Campus Mare Nostrum”, University of Murcia, E-30100 Murcia, Spain
Manuel Hernández-Córdoba
Department of Analytical Chemistry, Faculty of Chemistry, Regional Campus of International Excellence “Campus Mare Nostrum”, University of Murcia, E-30100 Murcia, Spain
Natalia Arroyo-Manzanares
Department of Analytical Chemistry, Faculty of Chemistry, Regional Campus of International Excellence “Campus Mare Nostrum”, University of Murcia, E-30100 Murcia, Spain
Pilar Viñas
Department of Analytical Chemistry, Faculty of Chemistry, Regional Campus of International Excellence “Campus Mare Nostrum”, University of Murcia, E-30100 Murcia, Spain
Nitrosamines (NAs), which are catalogued as carcinogenic compounds, may be present in meat products due to the conversion of nitrites and as result of migration from elastic rubber nettings used. A method based on ultrasonic assisted extraction coupled with dispersive liquid–liquid microextraction as sample treatment and gas chromatography-mass spectrometry as separation and detection technique was proposed for the determination of twelve NAs in cooked ham samples. The method was validated by evaluating linearity (0.5–1000 ng g−1), matrix effect, sensitivity (detection limits were between 0.15 and 1.4 ng g−1) and precision, which was below 12%. Five NAs were found in the samples with levels ranging from not quantifiable to 40 ng g−1. The effect of the elastic rubber nettings on the nitrosamine content of meat was evaluated by comparing the levels found in products made with several plastics or thread in the presence of additives.
