Proteomic and glycomic analyses of a lung-specific protein surfactant protein-D
Emi Ito,
Ritsuko Oka,
Takeo Ishii,
Hiroaki Korekane,
Ayako Kurimoto,
Yasuhiko Kizuka,
Shinobu Kitazume,
Shigeru Ariki,
Motoko Takahashi,
Yoshio Kuroki,
Kozui Kida,
Naoyuki Taniguchi
Affiliations
Emi Ito
Diease Glycomics Team, Systems Glycobiology Research Group, RIKEN-Max Planck Joint Research Center, RIKEN Global Research Cluster, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan
Ritsuko Oka
Diease Glycomics Team, Systems Glycobiology Research Group, RIKEN-Max Planck Joint Research Center, RIKEN Global Research Cluster, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan
Takeo Ishii
Respiratory Care Clinic, Nippon Medical School, 4-7-15-8F Kudan-Minami, Chiyoda-ku, Tokyo 102-0074, Japan
Hiroaki Korekane
Diease Glycomics Team, Systems Glycobiology Research Group, RIKEN-Max Planck Joint Research Center, RIKEN Global Research Cluster, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan
Ayako Kurimoto
Diease Glycomics Team, Systems Glycobiology Research Group, RIKEN-Max Planck Joint Research Center, RIKEN Global Research Cluster, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan
Yasuhiko Kizuka
Diease Glycomics Team, Systems Glycobiology Research Group, RIKEN-Max Planck Joint Research Center, RIKEN Global Research Cluster, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan
Shinobu Kitazume
Diease Glycomics Team, Systems Glycobiology Research Group, RIKEN-Max Planck Joint Research Center, RIKEN Global Research Cluster, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan
Shigeru Ariki
Department of Biochemistry, Sapporo Medical University School of Medicine, Sapporo 060-8556, Japan
Motoko Takahashi
Department of Biochemistry, Sapporo Medical University School of Medicine, Sapporo 060-8556, Japan
Yoshio Kuroki
Department of Biochemistry, Sapporo Medical University School of Medicine, Sapporo 060-8556, Japan
Kozui Kida
Respiratory Care Clinic, Nippon Medical School, 4-7-15-8F Kudan-Minami, Chiyoda-ku, Tokyo 102-0074, Japan
Naoyuki Taniguchi
Diease Glycomics Team, Systems Glycobiology Research Group, RIKEN-Max Planck Joint Research Center, RIKEN Global Research Cluster, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan
In order to verify the protein enriched from pooled human sera to be a lung-specific protein surfactant protein-D (SP-D), we performed peptide mass fingerprinting (PMF)-based protein identification. MASCOT search results of the obtained PMF unequivocally demonstrated that it is identical to human SP-D. Meanwhile, we performed MALDI-QIT-TOF mass spectrometry-based N-glycomic analysis of the recombinant human SP-D produced in murine myeloma cells. The obtained mass spectra of N-glycans from the recombinant SP-D demonstrated that the recombinant protein is almost exclusively modified with core-fucosylated N-glycans [1].
