Bioactive Materials (Apr 2024)
Photonic control of ligand nanospacing in self-assembly regulates stem cell fate
- Sungkyu Lee,
- Jounghyun Yoo,
- Gunhyu Bae,
- Ramar Thangam,
- Jeongyun Heo,
- Jung Yeon Park,
- Honghwan Choi,
- Chowon Kim,
- Jusung An,
- Jungryun Kim,
- Kwang Rok Mun,
- Seungyong Shin,
- Kunyu Zhang,
- Pengchao Zhao,
- Yuri Kim,
- Nayeon Kang,
- Seong-Beom Han,
- Dahee Kim,
- Jiwon Yoon,
- Misun Kang,
- Jihwan Kim,
- Letao Yang,
- Solmaz Karamikamkar,
- Jinjoo Kim,
- Yangzhi Zhu,
- Alireza Hassani Najafabadi,
- Guosheng Song,
- Dong-Hwee Kim,
- Ki-Bum Lee,
- Soong Ju Oh,
- Hyun-Do Jung,
- Hyun-Cheol Song,
- Woo Young Jang,
- Liming Bian,
- Zhiqin Chu,
- Juyoung Yoon,
- Jong Seung Kim,
- Yu Shrike Zhang,
- Yongju Kim,
- Ho Seong Jang,
- Sehoon Kim,
- Heemin Kang
Affiliations
- Sungkyu Lee
- Department of Materials Science and Engineering, Korea University, Seoul, 02841, Republic of Korea
- Jounghyun Yoo
- Chemical and Biological Integrative Research Center, Korea Institute of Science and Technology, Seoul, 02792, Republic of Korea
- Gunhyu Bae
- Department of Materials Science and Engineering, Korea University, Seoul, 02841, Republic of Korea
- Ramar Thangam
- Department of Materials Science and Engineering, Korea University, Seoul, 02841, Republic of Korea
- Jeongyun Heo
- Chemical and Biological Integrative Research Center, Korea Institute of Science and Technology, Seoul, 02792, Republic of Korea
- Jung Yeon Park
- KU-KIST Graduate School of Converging Science and Technology, Korea University, Seoul, 02841, Republic of Korea
- Honghwan Choi
- Chemical and Biological Integrative Research Center, Korea Institute of Science and Technology, Seoul, 02792, Republic of Korea; KU-KIST Graduate School of Converging Science and Technology, Korea University, Seoul, 02841, Republic of Korea
- Chowon Kim
- Department of Materials Science and Engineering, Korea University, Seoul, 02841, Republic of Korea
- Jusung An
- Department of Chemistry, Korea University, Seoul, 02841, Republic of Korea
- Jungryun Kim
- Department of Chemistry, Korea University, Seoul, 02841, Republic of Korea
- Kwang Rok Mun
- Materials Architecturing Research Center, Korea Institute of Science and Technology, Seoul, 02792, Republic of Korea
- Seungyong Shin
- Materials Architecturing Research Center, Korea Institute of Science and Technology, Seoul, 02792, Republic of Korea
- Kunyu Zhang
- School of Biomedical Sciences and Engineering, Guangzhou International Campus, South China University of Technology, Guangzhou, 511442, China
- Pengchao Zhao
- School of Biomedical Sciences and Engineering, Guangzhou International Campus, South China University of Technology, Guangzhou, 511442, China
- Yuri Kim
- Department of Materials Science and Engineering, Korea University, Seoul, 02841, Republic of Korea
- Nayeon Kang
- Department of Materials Science and Engineering, Korea University, Seoul, 02841, Republic of Korea
- Seong-Beom Han
- KU-KIST Graduate School of Converging Science and Technology, Korea University, Seoul, 02841, Republic of Korea
- Dahee Kim
- Department of Materials Science and Engineering, Korea University, Seoul, 02841, Republic of Korea
- Jiwon Yoon
- Chemical and Biological Integrative Research Center, Korea Institute of Science and Technology, Seoul, 02792, Republic of Korea
- Misun Kang
- Materials Architecturing Research Center, Korea Institute of Science and Technology, Seoul, 02792, Republic of Korea
- Jihwan Kim
- Chemical and Biological Integrative Research Center, Korea Institute of Science and Technology, Seoul, 02792, Republic of Korea; KU-KIST Graduate School of Converging Science and Technology, Korea University, Seoul, 02841, Republic of Korea
- Letao Yang
- Department of Chemistry and Chemical Biology, Rutgers University, Piscataway, NJ, 08854, USA
- Solmaz Karamikamkar
- Terasaki Institute for Biomedical Innovation, Los Angeles, CA, 90064, USA
- Jinjoo Kim
- Terasaki Institute for Biomedical Innovation, Los Angeles, CA, 90064, USA
- Yangzhi Zhu
- Terasaki Institute for Biomedical Innovation, Los Angeles, CA, 90064, USA
- Alireza Hassani Najafabadi
- Terasaki Institute for Biomedical Innovation, Los Angeles, CA, 90064, USA
- Guosheng Song
- State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, China
- Dong-Hwee Kim
- KU-KIST Graduate School of Converging Science and Technology, Korea University, Seoul, 02841, Republic of Korea
- Ki-Bum Lee
- Department of Chemistry and Chemical Biology, Rutgers University, Piscataway, NJ, 08854, USA
- Soong Ju Oh
- Department of Materials Science and Engineering, Korea University, Seoul, 02841, Republic of Korea
- Hyun-Do Jung
- Department of Biomedical-Chemical Engineering, The Catholic University of Korea, Gyeonggi-do, 14662, Republic of Korea; Division of Materials Science and Engineering, Hanyang University, Seoul, 04763, Republic of Korea
- Hyun-Cheol Song
- Electronic Materials Research Center, Korea Institute of Science and Technology, Seoul, 02792, Republic of Korea; KIST-SKKU Carbon-Neutral Research Center, Sungkyunkwan University (SKKU), Suwon, 16419, Republic of Korea
- Woo Young Jang
- Department of Orthopedic Surgery, Korea University Anam Hospital, Seoul, 02841, Republic of Korea
- Liming Bian
- School of Biomedical Sciences and Engineering, Guangzhou International Campus, South China University of Technology, Guangzhou, 511442, China
- Zhiqin Chu
- Department of Electrical and Electronic Engineering and Joint Appointment with School of Biomedical Sciences, The University of Hong Kong, Hong Kong, 518057, China
- Juyoung Yoon
- Department of Chemistry and Nanoscience, Ewha Womans University, Seoul, 03760, Republic of Korea
- Jong Seung Kim
- Department of Chemistry, Korea University, Seoul, 02841, Republic of Korea
- Yu Shrike Zhang
- Division of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital Harvard Medical School, Cambridge, MA, 02139, USA
- Yongju Kim
- KU-KIST Graduate School of Converging Science and Technology, Korea University, Seoul, 02841, Republic of Korea
- Ho Seong Jang
- Materials Architecturing Research Center, Korea Institute of Science and Technology, Seoul, 02792, Republic of Korea; Division of Nano & Information Technology, KIST School, Korea University of Science and Technology (UST), Seoul, 02792, Republic of Korea; Corresponding author. Materials Architecturing Research Center, Korea Institute of Science and Technology, Seoul, 02792, Republic of Korea.
- Sehoon Kim
- Chemical and Biological Integrative Research Center, Korea Institute of Science and Technology, Seoul, 02792, Republic of Korea; KU-KIST Graduate School of Converging Science and Technology, Korea University, Seoul, 02841, Republic of Korea; Corresponding author. Chemical and Biological Integrative Research Center, Korea Institute of Science and Technology, Seoul, 02792, Republic of Korea.
- Heemin Kang
- Department of Materials Science and Engineering, Korea University, Seoul, 02841, Republic of Korea; College of Medicine, Korea University, Seoul, 02841, Republic of Korea; Corresponding author. Department of Materials Science and Engineering, Korea University, Seoul, 02841, Republic of Korea.
- Journal volume & issue
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Vol. 34
pp. 164 – 180
Abstract
Extracellular matrix (ECM) undergoes dynamic inflation that dynamically changes ligand nanospacing but has not been explored. Here we utilize ECM-mimicking photocontrolled supramolecular ligand-tunable Azo+ self-assembly composed of azobenzene derivatives (Azo+) stacked via cation-π interactions and stabilized with RGD ligand-bearing poly(acrylic acid). Near-infrared-upconverted-ultraviolet light induces cis-Azo+-mediated inflation that suppresses cation-π interactions, thereby inflating liganded self-assembly. This inflation increases nanospacing of “closely nanospaced” ligands from 1.8 nm to 2.6 nm and the surface area of liganded self-assembly that facilitate stem cell adhesion, mechanosensing, and differentiation both in vitro and in vivo, including the release of loaded molecules by destabilizing water bridges and hydrogen bonds between the Azo+ molecules and loaded molecules. Conversely, visible light induces trans-Azo+ formation that facilitates cation-π interactions, thereby deflating self-assembly with “closely nanospaced” ligands that inhibits stem cell adhesion, mechanosensing, and differentiation. In stark contrast, when ligand nanospacing increases from 8.7 nm to 12.2 nm via the inflation of self-assembly, the surface area of “distantly nanospaced” ligands increases, thereby suppressing stem cell adhesion, mechanosensing, and differentiation. Long-term in vivo stability of self-assembly via real-time tracking and upconversion are verified. This tuning of ligand nanospacing can unravel dynamic ligand-cell interactions for stem cell-regulated tissue regeneration.
